TREM-1 A811G基因多态性与慢性乙型肝炎感染的相关性分析  

Correlation analysis of TREM-1 A811G gene polymorphism with chronic hepatitis B infection

作　　者：戴显宁 王倩[2] 项心怡 童郁 许锴 DAI Xian-ning;WANG Qian;XIANG Xin-yi;TONG Yu;XU Kai(Clinical Laboratory,Wenzhou People's Hospital,Zhejiang 325000,China;不详)

机构地区：[1]温州市人民医院检验科,浙江325000 [2]温州医科大学检验与生命科学学院

出　　处：《中国卫生检验杂志》2023年第21期2622-2625,共4页Chinese Journal of Health Laboratory Technology

基　　金：温州市基础性医疗卫生科技项目(Y2020572)。

摘　　要：目的探讨TREM-1A811G基因多态性在CHB发生发展过程中与炎性反应的相关性。方法运用Sanger测序对TREM-1基因测序后,分析TREM-1基因多态性在CHB组及其亚组和对照组中基因型、等位基因频率及危险度(OR)计算,评估TREM-1基因多态性位点在CHB感染中与炎症反应的相关性。结果果TREM-1A811G基因突变位点在CHB组和对照组中均检测出AA、AG、GG3种基因型,其中在CHB组中AG基因型频率最高(41.13%,167/406);健康对照组中AA基因型频率最高(36.67%,33/90)。且CHB组G等位基因频率高于对照组(Х^(2)=5.555,P<0.05),G等位基因相对于A等位基因(OR)为1.475(95%CI:1.066~2.039)。CHB组3种不同基因型中,其中ALT血清水平差异有统计学意义(P<0.05);而TBIL和AST血清水平差异无统计学意义(P>0.05)。结论TREM-1A811G基因型及分布频率在CHB组和对照组中分布有差异,携带A等位基因的人群相比较携带G等位基因人群CHB的感染炎性反应程度风险相对较低,可能是影响慢性肝炎进展及易感性的因素之一。Objective This paper aims to investigate the correlation between TREM-1A811G gene polymorphism and inflam-matory response in the development and progression of chronic hepatitis B(CHB).Methods After Sanger sequencing,the genotypes,allele frequency and risk OR of TREM-1 gene polymorphism were analyzed between CHB group and control group.And the association of TREM-1 with inflammation in CHB infection was evaluated.Results AA,AG,GG genotypes at TREM-1 A811G gene mutation site were detected in CHB group and control group.The frequency of AG genotype in CHB group was the highest(41.13%,167/406).The frequency of AA genotype was the highest in the control group(36.67%,33/90).The frequency of G allele in CHB group was higher than that in the control group(χ^(2)=5.555,P<0.05),and the risk(OR)of G allele relative to A allele was 1.475(95%CI:1.066-2.039).Among the three different genotypes in CHB group,there was statistical significance on the difference in the serum level of ALT(P<0.05).There was no statistical signifi-cance on the difference in serum levels between TBIL and AST(P>0.05).Conclusion The distribution and frequency of TREM-1 A811G genotype are different between CHB group and control group.Compared with the G allele-carrying population,the A allele-carrying population has a lower risk of inflammatory response degree of CHB,which may be one of the factors affecting the progression and susceptibility of CHB.

关 键 词：慢性乙型病毒性肝炎 髓样细胞触发受体-1 基因多态性 炎性反应 感染 

分 类 号：R512.62[医药卫生—内科学]