电子束辐照对不同载体上两种微生物的消毒效果研究  

Study on the disinfection effect of electron beam irradiation on two kinds of microorganisms on different carriers

作　　者：陈妙玲 徐玉清 胡璐璐 王磊 罗桂贤 邱亚群[1] 方泽坤 钟娥秋 刘宵宵 兰文升 扈庆华[1] 李迎慧[1] CHEN Miao-ling;XU Yu-qing;HU Lu-lu;WANG Lei;LUO Gui-xian;QIU Ya-qun;FANG Ze-kun;ZHONG E-qiu;LIU Xiao-xiao;LAN Wen-sheng;HU Qing-hua;LI Ying-hui(Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong 518073,China;不详)

机构地区：[1]深圳市疾病预防控制中心,广东深圳518073 [2]中广核金沃科技有限公司 [3]深圳海关动植物检验检疫技术中心

出　　处：《现代预防医学》2023年第21期3965-3968,3990,共5页Modern Preventive Medicine

基　　金：海关总署科研项目(2021HK167);深圳市科技计划项目(JSGG20220301085800001);深圳市引进高层次医学团队项目“三名工程”(SZSM201811071)。

摘　　要：目的 研究电子束射线对不同载体上白色念珠菌和短小杆菌芽孢的消毒效果,探讨电子束辐照在消毒领域的应用价值。方法 在不同材质载体上以白色念珠菌、短小杆菌芽孢为指示微生物制作染菌载体,用2~6 k Gy工艺剂量的电子束射线辐照,计算杀灭对数值。选各种染菌载体杀灭对数值均≥3.00的最小工艺剂量做模拟现场实验,评价其消毒效果。结果 电子束辐照剂量达到≥4 k Gy时,对聚四氟乙烯片、玻璃片、不锈钢片上的白色念珠菌和布片、不锈钢片上的短小杆菌芽孢的平均杀灭对数值≥3.00;当工艺剂量达到≥5 k Gy时,对牛皮纸片载体上的白色念珠菌和聚四氟乙烯片、玻璃片、牛皮纸片上的短小杆菌芽孢的平均杀灭对数值≥3.00。模拟现场试验中,电子束辐照工艺剂量达到5.12~5.54 k Gy时,对四种载体上的白色念珠菌和五种载体上的短小杆菌芽孢的的平均杀灭对数值均≥3.00。结论 电子束辐照剂量达到≥5 k Gy时,对不同载体上的真菌和辐照指示微生物有较好的消毒效果。电子束辐照穿透式消毒要综合考虑内容物规格、密度差异等,选择合适的辐照剂量。Objective To investigate the effect of electron beam irradiation on the disinfection of Candida albicans and Bacillus poilus spores on different carriers and to explore the application value of electron beam irradiation in the field of disinfection.Methods Candida albicans and Bacillus pummelos spores were used as indicator microorganisms to make infection carriers on different material carriers,and the killing logarithm was calculated by electron beam irradiation of 2 to 6kGy process dose.The minimum process dose of all kinds of infection carriers with killing logarithm≥3.00 was selected for simulated field experiment,and the disinfection efficacy was evaluated.Results The average killing logarithm value of Candida albicans on polytetrafluoroethylene sheet,glass sheet,and stainless-steel sheet and Bacillus pummelos spores on cloth and stainless-steel strip was≥3.00 when the electron beam irradiation dose was≥4kGy,and≥3.00 on Kraft paper carrier when the process dose was≥5kGy.In the simulated field test,when the dose of electron beam irradiation reached 5.12 to 5.54kGy,the average killing logarithm values of Candida albicans on four carriers and Bacillus pummelos spores on five carriers were all≥3.00.Conclusion When the dose of electron beam irradiation reaches≥5kGy,it has a good disinfection effect on fungi and radiation indicator microorganisms on different carriers.Electron beam irradiation penetrating disinfection should comprehensively consider the difference of content specification and density and select the appropriate irradiation dose.

关 键 词：电子束辐照 消毒效果 短小杆菌芽孢 白色念珠菌 

分 类 号：R187[医药卫生—流行病学]