CuNCs荧光探针检测环境水样中的孔雀石绿  

CuNCs fluorescent probe for the detection of malachite green in environmental water samples

作　　者：刘梅 林洪 吴云英 胡栋宝 伍贤学 余琴 陈文静 谢建新 LIU Mei;LIN Hong;WU Yunying;HU Dongbao;WU Xianxue;YU Qin;CHEN Wenjing;XIE Jianxin(School of Chemistry,Biology and Environment,Yuxi Normal University,Yuxi 653100,China;School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalites,Duyun 55800,China)

机构地区：[1]玉溪师范学院化学生物与环境学院,玉溪653100 [2]黔南民族师范学院化学化工学院,都匀558000

出　　处：《分析试验室》2023年第10期1279-1284,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21465024);云南省地方本科高校(部分)基础研究联合专项(2019FH001(-112));云南省高校科技创新团队;玉溪师范学院大学生创新创业训练项目(202111390039,2021A010,2019B19);黔南民族师范学院高层次引进人才研究专项项目资助。

摘　　要：以Cu(NO_(3))_(2)为原料,D-青霉胺(DPA)为还原剂和稳定剂,采用一锅法在室温下制备出高量子产率的红色荧光铜纳米簇(CuNCs),构建了一种可简单快速检测孔雀石绿(MG)的荧光探针。优化了CuNCs的合成条件,采用透射电子显微镜(TEM)、X射线光电子能谱(XPS)、傅里叶变换红外光谱(FT-IR)对CuNCs的形貌及组成进行了表征。MG对CuNCs具有选择性猝灭效应,在1~75μmol/L浓度范围内,MG的浓度与CuNCs的荧光强度(F0/F)呈良好的线性关系,检出限为0.75μmol/L。该荧光探针成功用于湖水中MG的灵敏检测,加标回收率为99.1%~109.9%。该方法可用于环境水样中MG的高灵敏检测。Using copper nitrate as raw material and D-penicillamine(DPA) as reducing and stabilizing agent,red fluorescent copper nanoclusters(CuNCs) with high quantum yield were prepared by one-pot method at room temperature,and then a fluorescent probe for simple and rapid detection of malachite green(MG) was established.The synthesis conditions were optimized,and the morphology and composition of CuNCs were characterized by transmission electron microscope(TEM),X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectrometer(FT-IR).The fluorescence of CuNCs could be selectively quenched by MG.The fluorescence intensity of CuNCs(F0/F) had a good linear relationship with the concentration of MG in the range of 1-75 μmol/L,and the detection limit was as low as 0.75 μmol/L.The fluorescence probe was successfully used for the sensitive detection of MG in lake water,and the recoveries were 99.1%-109.9%.The proposed method could be applied for highly sensitive detection of MG in environmental water samples.

关 键 词：铜纳米簇 孔雀石绿 荧光猝灭 

分 类 号：O657.3[理学—分析化学]