超声法制备环糊精-铂纳米酶并用于检测葡萄糖  

Ultrasound-assisted synthesis of β-cyclodextrin-platinum nanozyme and its application for glucose detection

作　　者：姜翠凤[1,2] 王金山 阮志凡 熊芳芳 刘星宇 刘艺媛 郭伟 JIANG Cuifeng;WANG Jinshan;RUAN Zhifan;XIONG Fangfang;LIU Xingyu;LIU Yiyuan;GUO Wei(School of Materials Science and Engineering,Yancheng Institute of Technology,Yancheng 224051,China;Key Laboratory for Ecological-Environment Materials of Jiangsu Province,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]盐城工学院材料科学与工程学院,盐城224051 [2]盐城工学院江苏省生态环境材料重点实验室,盐城224051

出　　处：《分析试验室》2023年第10期1304-1310,共7页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21605128,51872252)项目资助。

摘　　要：通过超声辅助法制备了具有类过氧化物酶、类氧化物酶和类过氧化氢酶活性的β-环糊精-铂纳米粒子(CD-PtNPs),该纳米材料在同一酸度条件下同时具有3种类酶活性。对影响其类过氧化物酶活性的因素进行了优化,最佳条件为:温度20℃,pH 3.6,3,3’,5,5’-四甲基联苯胺(TMB)浓度2 mmol/L,H2O2浓度78μmol/L,CD-PtNPs用量0.648μg。基于其类过氧化物酶活性,构建了可通过比色和紫外可见吸收光谱检测葡萄糖的传感平台。该传感平台在缓冲溶液中对葡萄糖的检出限为80μmol/L,在30%(V/V)血清中检出限为0.18 mmol/L。CD-PtNPs制备方法简便、快速且可避免聚集,有望在生物分析领域得到广泛应用。cyclodextrin-platinum nanoparticles(CD-PtNPs) with peroxidase-,oxidase-and catalase-like activity were prepared by an ultrasound-assisted reduction method.The optimal conditions for peroxidase-like activity were as follows:20 ℃,pH 3.6,2 mmol/L of TMB,78 μmol/L of H2O2 and 0.648 μg of CD-PtNPs.Based on the excellent peroxidase-like activity,the limit of detection(LOD) for glucose was of 80 μmol/L.Furthermore,this method allowed rapid detection of glucose in 30%(V/V) serum with LOD of 0.18 mmol/L.This ultrasoundassisted method could be expanded to prepare other kind of nanozymes,and CD-PtNPs were expected to have wide application in bioanalytical chemistry.

关 键 词：超声辅助合成 纳米酶 铂纳米材料 葡萄糖 传感器 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]