A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation  

作　　者：LIAO Guang-lian HUANG Chun-hui JIA Dong-feng ZHONG Min TAO Jun-jie QU Xue-yan XU Xiao-biao 

机构地区：[1]Institute of Kiwifruit,Jiangxi Agricultural University,Nanchang 330045,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第11期3244-3255,共12页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China (32160692 and 31760559);the Key Research and Development Plan from Jiangxi Science and Technology Department, China (20192ACB60002)。

摘　　要：Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content.However, the molecular mechanism driving AsA richness in fruit remains unclear.In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory network related to AsA metabolism by combining genomics,metabolomics and transcriptomics.We assembled a high-quality genome of A. eriantha ‘Ganlv 1’ with only five remaining gaps.The assembly is comprised of 29 pseudochromosomes with a total size of 615.95 Mb, and contig N50 of 20.35 Mb.Among them,24 of the pseudochromosomes were obtained directly from telomere-to-telomere.The LTR assembly index score and consensus quality value were 21.34 and 39.90%, respectively.Subsequently, 61 metabolites and 2 092genes were found to be differentially accumulated/expressed during fruit development by metabolome and transcriptome assays, respectively.AsA metabolism and the cyclic regeneration pathway were found to have high expression levels throughout fruit growth and development,suggesting its crucial role in the regulation of AsA.Furthermore,the AsA contents are highly associated with ascorbate peroxidase genes.The genome obtained in this study provides genomic resources for the genetic and breeding research ofA. eriantha, and the constructed regulatory network can provide a public data platform for future research on kiwifruit.

关 键 词：Actinidia eriantha ascorbic acid non-targeted metabolome gapless genome TRANSCRIPTOME 

分 类 号：S663.4[农业科学—果树学]