Development and application of KASP marker for high throughput detection of the seedless trait in grapevine  

作　　者：WANG Fu-qiang BIAN Lu QIU Peng-peng GUO Shuo GUO Jing-han GUO Chen-shuo JIANG Jian-fu LIU Chong-huai WANG Yong LIU Guo-tian WANG Yue-jin XU Yan 

机构地区：[1]Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture and Rural Affairs/State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China [2]Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,P.R.China [3]Research Institute of Grape and Melon of Xinjiang Uygur Autonomous Region,Shanshan 838200,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第11期3269-3283,共15页农业科学学报（英文版）

基　　金：supported by the earmarked fund for the China Agriculture Research System of MOF and MARA (CARS-29-yc-3);the Project of Agricultural Breeding in Ningxia Hui Autonomous Region,China (NXNYYZ20210104);the Key Industrial Innovation Chain Project in Shaanxi Province,China(2021ZDLNY04-08)。

摘　　要：Molecular marker-assisted selection(MAS) can significantly accelerate and improve the efficiency of the breeding process in seedless grape cultivars. In this study, we developed the KASP_VviAGL11 and VviAGL11_410 markers based on a single nucleotide polymorphism(SNP) site(Chr18: 26889437(A/C)) of the VviAGL11 gene, and compared them with previously reported SSR markers p3_VvAGL11 and 5U_VviAGL11 by testing 101 cultivars and 81 F1 hybrid progenies. The results showed that both of the proposed markers obtained 100% accuracy rates in detecting allele A, which was closely associated with the seedless trait in grapes, while p3_VvAGL11 and 5U_VviAGL11 had lower accuracy rates due to their tendency to produce false positives. After careful evaluation of the technical advantages and disadvantages associated with these markers, we concluded that KASP_VviAGL11 was superior in terms of simplicity,cost-effectiveness, efficiency, and accuracy. Thus, we optimized the process of molecular MAS for seedless grapes,focusing on the KASP_VviAGL11 marker as a central component, to provide key technical support for the development of new seedless grape cultivars.

关 键 词：seedless grape MAS KASP SSR 

分 类 号：S663.1[农业科学—果树学]