QTL analysis of early flowering of female flowers in zucchini(Cucurbita pepo L.)  

作　　者：QU Shu-ping YANG Dan YU Hai-yang CHEN Fang-yuan WANG Ke-xin DING Wen-qi XU Wen-long WANG Yun-li 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs/College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,P.R.China [2]Laboratory of Molecular Breeding of Pumpkin,College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第11期3321-3330,共10页农业科学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China (32072590 and 32002051);the China Postdoctoral Science Foundation (2019M661244);the Academic Backbone Foundation of Northeast Agricultural University, China (20XG03)。

摘　　要：Early flowering promotes early maturity, production, and the capacity to counteract biotic and abiotic stresses, making it an important agronomic trait in zucchini. The present study demonstrated that the zucchini inbred line ‘19’ consistently flowered early, taking significantly fewer days to bloom the first female flower(DFF) than the inbred line ‘113’. Genetic analysis revealed that DFF, an inheritable quantitative trait, is controlled by multiple genes. Based on the strategy of quantitative trait locus(QTL) sequencing(QTL-seq) combined with linkage analysis, three QTLs for DFF were identified on chromosomes 4, 11, and 20. This study used additional F2 populations grown under different environmental conditions for QTL mapping analysis of DFF with insertion/deletion(InDel) markers to validate these results. Using the composite interval mapping(CIM) method of R/qtl software, we only identified one major locus under all environmental conditions, located in a 117-kb candidate region on chromosome 20. Based on gene annotation, gene sequence alignment, and qRT-PCR analysis, we found that the Cp4.1LG20g08050 gene encoding a RING finger protein may be a candidate gene for the opposite regulation of early flowering in zucchini. In summary, these results lay a foundation for a better understanding of early flowering and improving early flowering-based breeding strategies in zucchini.

关 键 词：Cucurbita pepo L. early flowering days to bloom the first female flower QTL analysis 

分 类 号：S642.6[农业科学—蔬菜学]