西洋参总皂苷通过Nrf2-ARE通路对压力性尿失禁的治疗作用  

Therapeutic Effects of American Ginseng Total Saponins on Stress Urinary Incontinence via Modulation of the Nrf2-ARE Pathway

作　　者：杨丹丹[1] 唐红英 张蝶 魏国斌[1] 张靖雪 李南希 YANG Dandan;TANG Hongying;ZHANG Die(Guang'an People's Hospital,Sichuan Guang'an 638000,China)

机构地区：[1]四川省广安市人民医院妇科,四川广安638000

出　　处：《河北医学》2023年第11期1805-1811,共7页Hebei Medicine

基　　金：四川省卫生和计划生育委员会2018年科研课题项目,(编号:18PJ042)。

摘　　要：目的:探究西洋参总皂苷通过调控Nrf2/ARE通路对压力性尿失禁的治疗作用。方法:制备压力性尿失禁大鼠模型,将60只大鼠随机分为对照组(Control组)、压力性尿失禁大鼠模型组(SUI组)、压力性尿失禁大鼠模型分别给予低、中、高剂量西洋参总皂苷干组(PQSL组、PQSM组、PQSH组)和PQSH组基础上给予Nrf2特异性抑制剂ML385干预组(PQSH+ML385组),每组10只。统计各组大鼠喷嚏阳性率;测定尿动力学指标(MBC、LPP、ALPP);测定膀胱内压(排尿量、残余尿量、排尿效率);Masson染色检测尿道组织胶原纤维量;检测尿道组织氧化应激指(MDA、SOD、GSH-Px);蛋白质印迹检测脑组织中Nrf2、HO-1、NOQ1蛋白表达。结果:Control组大鼠喷嚏实验均为阴性。造模50只大鼠均出现了喷嚏实验阳性;PQSL组、PQSM组、PQSH组喷嚏实验阳性率分别为60%(6/10)、40%(4/10)、20%(2/10),PQSH+ML385组大鼠喷嚏实验阳性率为70%(7/10),SUI组大鼠喷嚏实验阳性率为80%(8/10)。和Control组相比,SUI组大鼠MBC、LPP、ALPP水平、排尿效率、GSH-Px含量和SOD活性降低,排尿量、残余尿量、MDA含量增多(P<0.05);和SUI组相比,PQSL组、PQSM组、PQSH组大鼠MBC、LPP、ALPP水平、排尿效率、GSH-Px含量和SOD活性增加,排尿量、残余尿量、MDA含量减少,且呈浓度依赖(P<0.05);和PQSH组相比,PQSH+ML385组大鼠MBC、LPP、ALPP水平、排尿效率、GSH-Px含量和SOD活性降低,排尿量、残余尿量、MDA含量增多(P<0.05)。和Control组相比,SUI组大鼠尿道组织中Nrf2、HO-1、NQO1蛋白表达明显降低(P<0.05);和SUI组相比,PQSL组、PQSM组、PQSH组大鼠尿道组织中Nrf2、HO-1、NQO1蛋白表达均明显升高,且呈剂量依赖(P<0.05);和PQSH组相比,PQSH+ML385组大鼠尿道组织中Nrf2、HO-1、NQO1蛋白表达均明显降低(P<0.05)。结论:西洋参总皂苷可通过激活Nrf2/ARE信号通路改善压力性尿失禁排尿功能,从而对压力性尿失禁达到治疗作用。Objective:To explore the therapeutic effect of total saponins of American ginseng on stress urinary incontinence by regulating Nrf2/ARE pathway.Methods:A stress urinary incontinence rat model was established.Sixty rats were randomly assigned to the following groups:Control group,Stress Urinary Incontinence(SUI)group,and SUI groups treated with low,medium,and high doses of American Ginseng Total Saponins(PQSL,PQSM,PQSH).Additionally,one SUI group received Nrf2-specific inhibitor ML385(PQSH+ML385).Each group consisted of 10 rats.The positive sneezing rates were recorded.Urodynamic parameters,including MBC,LPP,ALPP,as well as intravesical pressure(voided volume,residual volume,voiding efficiency)were measured.Masson staining was used to assess collagen fiber levels in urethral tissues.Oxidative stress indicators(MDA,SOD,GSH-Px)were determined in urethral tissues.Western blot analysis was performed to assess the expression of Nrf2,HO-1,and NQO1 in brain tissue.Results:In the Control group,sneezing tests were consistently negative.In the SUI group,all 50 rats showed positive sneezing test results.The positive sneezing rates in PQSL,PQSM,and PQSH groups were 60%(6/10),40%(4/10),and 20%(2/10),respectively,whereas in the PQSH+ML385 group,the rate was 70%(7/10),and in the SUI group,it was 80%(8/10).Compared to the Control group,the SUI group exhibited reduced MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,along with increased urine volume,residual volume,and MDA content(P<0.05).In comparison with the SUI group,the PQSL,PQSM,and PQSH groups demonstrated elevated MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,and reduced urine volume,residual volume,and MDA content,with effects showing concentration-dependent patterns(P<0.05).When compared to the PQSH group,the PQSH+ML385 group displayed decreased MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,alongside increased urine volume,residual volume,and MDA content(P<0.05).Compared to the Control group,the SUI

关 键 词：西洋参总皂苷 压力性尿失禁 排尿功能 Nrf2/ARE信号 

分 类 号：R285.5[医药卫生—中药学]