补肾中药佳蓉片治疗大鼠宫腔粘连的有效性研究  

Efficacy of Jiarong Tablets in Treating Intrauterine Adhesions in Rats

作　　者：梁宇同 王研 丁旭 李臻 师长宏 黄艳红 LIANG Yu-tong;WANG Yan;DING Xu;LI Zhen;SHI Chang-hong;HUANG Yan-hong(The First Clinical Medical College of Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046;Xi′an In-ternational Medical Center Hospital,Xi′an Shaanxi 710100;Air Force Medical University,Xi′an Shaanxi 710032)

机构地区：[1]陕西中医药大学第一临床医学院,陕西咸阳712046 [2]西安国际医学中心医院,陕西西安710100 [3]空军军医大学,陕西西安710032

出　　处：《世界中西医结合杂志》2023年第9期1756-1760,1786,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：陕西省重点研发计划项目产业创新链课题(2020ZDLSF03-01)。

摘　　要：目的探讨不同剂量佳蓉片治疗大鼠宫腔粘连(Intrauterine adhesions,IUA)的有效性。方法SD大鼠行双重损伤法建立IUA模型,造模成功后将大鼠按随机数字表法分为模型组、雌激素组(于术后14 d、18 d肌肉注射苯甲酸雌二醇注射液)以及低、中、高浓度佳蓉片组(分别于术后3 d开始连续灌胃20 mg/ml、40 mg/ml、80 mg/ml佳蓉片28 d),每组各18只。另取18只SD大鼠作为对照组(仅查见双侧子宫)。术后33 d每组取材12只,HE染色评估宫腔形态、子宫内膜腺体情况,Masson染色评估子宫内膜纤维化程度,ELISA测定大鼠血清雌二醇(Estradiol,E2)含量,每组剩余的6只SD大鼠连续合笼一个动情周期,孕14d取材观察造模段妊娠情况。结果造模术后33 d,模型组见宫腔局部或全部闭塞、纤维粘连带形成,与对照组比较,腺体数量减少、规则形态腺体占比增加、纤维化面积比增加、血清E2含量降低,差异有统计学意义(P<0.01),且妊娠情况明显恶化。雌激素治疗组相较模型组,在子宫纤维化面积比、血清雌激素含量方面有所改善,而各剂量佳蓉片组与模型组比较,宫腔形态、纤维化面积比、血清E2含量均有明显改善,差异有统计学意义(P<0.01),且妊娠情况均有明显改善,中剂量佳蓉片治疗组腺体总数增加,差异有统计学意义(P<0.05)。结论补肾中药佳蓉片可有效治疗IUA,且中剂量佳蓉片的治疗效果最佳。Objective To investigate the efficacy of different doses of Jiarong Tablets in the treatment of intrauter⁃ine adhesions(IUAs)in rats.Methods The IUA model of SD rats was established by the double injury method.After suc⁃cessful modeling,the rats were assigned by the random number table method into model,estradiol benzoate(intramuscular injection on days 14 and 18 after surgery),and low-,medium-,and high-dose(20,40,and 80 mg/mL,respectively)Ji⁃arong Tablets(administrated by gavage since day 3 after surgery for 28 consecutive days)groups(n=18).Another 18 SD rats were selected as the control group(with only bilateral uterus examined).On day 33 after the modeling surgery,12 rats in each group were sampled.The uterine cavity morphology and endometrial glands were examined by hematoxylin-eosin(HE)staining,the degree of endometrial fibrosis by Masson staining,and the serum estradiol(E2)level by enzyme-linked immunosorbent assay.The remaining 6 rats in each group were subjected to consecutive caging for an estrous cycle and sampled on day 14 of pregnancy for observation of the gestation at the modeling stage.Results On day 33 after modeling,the model group showed partial or total uterine cavity occlusion,formation of fibrous adhesions,decreased glands,increased proportion of glands in the regular shape,increased proportion of fibrosis area,and lowered E2 level(P<0.01).Moreover,the pregnancy condition deteriorated significantly.Compared with the model group,estradiol benzoate decreased the propor⁃tion of fibrosis area and elevated E2 level,and different doses of Jiarong Tablets improved the uterine cavity morphology,de⁃creased the proportion of fibrosis area,and elevated serum E2 level(P<0.01).Moreover,the tablets improved the pregnan⁃cy condition,and the middle-dose Jiarong Tablets group presented increased glands(P<0.05).Conclusion The kidney-tonifying Chinese medicine Jiarong Tablets had a definite therapeutic effect on IUA,and the medium-dose Jiarong Tab⁃lets showed the best efficacy.

关 键 词：宫腔粘连 佳蓉片 子宫内膜纤维化 腺体 雌二醇 

分 类 号：R285.5[医药卫生—中药学]