妊娠期高血压疾病患者外周血miR-15-5P和妊娠相关血浆蛋白A水平动态监测的价值分析  

Value analysis of dynamic monitoring of peripheral blood microRNA-15-5P and pregnancy-associated plasma protein A levels in patients with hypertensive disorder complicating pregnancy

作　　者：蒋天从 戚桂杰[1] 谷孝月 刘志明 郭婉茹[1] 石冲[1] JIANG Tiancong;QI Guijie;GU Xiaoyue;LIU Zhiming;GUO Wanru;SHI Chong(Department of Prenatal Diagnosis,Tangshan Maternal and Child Health Hospital,Tangshan 063000,Hebei,China)

机构地区：[1]唐山市妇幼保健院产前诊断科,河北唐山063000

出　　处：《中国性科学》2023年第10期82-85,共4页Chinese Journal of Human Sexuality

基　　金：河北省医学科学研究课题计划项目(20221770)。

摘　　要：目的探讨动态监测外周血微小RNA-15-5P(miR-15-5P)和妊娠相关血浆蛋白A(PAPP-A)水平在妊娠期高血压疾病(HDCP)患者治疗中的临床价值。方法回顾性分析2019年7月至2021年6月于唐山市妇幼保健院就诊的165例HDCP患者的临床资料,纳入病例组,并根据病情严重程度分为妊娠高血压组(n=67)、轻度子痫前期组(n=63)、重度子痫前期组(n=35);选择同期在同一医院产检的91例正常妊娠女性作为对照组。所有研究对象均留取孕10、20、36周及产后1周的空腹静脉血,采用荧光定量聚合酶链反应(PCR)法测定miR-15-5P的表达水平,酶联免疫吸附试验(ELISA)法测定PAPP-A的血清含量。结果病例组和对照组的miR-15-5P水平均随着孕周的增加而降低,PAPP-A水平随着孕周的增加而升高,两组任意时间点的差异均具有统计学意义(P<0.05)。病例组在孕10、20、36周及产后1周的miR-15-5P水平均低于对照组,且妊娠高血压组、轻度子痫前期组、重度子痫前期组同一时间点依次降低,差异均具有统计学意义(P<0.05)。病例组在孕10、20、36周及产后1周的PAPP-A水平均高于对照组,且妊娠高血压组、轻度子痫前期组、重度子痫前期组同一时间点依次升高,差异均具有统计学意义(P<0.05)。结论动态监测外周血miR-15-5P和PAPP-A水平变化能够反映不同时期HDCP患者的严重程度,对判断该疾病的发生发展有一定的价值。Objective To explore the clinical value of dynamic monitoring of peripheral blood microRNA-15-5P(miR-15-5P)and pregnancy-associated plasma protein A(PAPP-A)in patients with hypertensive disorder complicating pregnancy(HDCP).Methods The clinical data of 165 patients with HDCP who were admitted to Tangshan Maternal and Child Health Hospital from July 2019 to June 2021 were retrospectively analyzed as the case group.According to the severity of the disease,they were divided into pregnancy induced hypertension group(n=67),mild preeclampsia group(n=63),and severe preeclampsia group(n=35).A total of 91 normal pregnant women who underwent prenatal examination in same hospital in the same period were selected as the control group.Fasting venous blood was collected at 10,20,36 weeks of gestation and 1 week after delivery.The expression level of miR-15-5P was determined by fluorescence quantitative polymerase chain reaction(PCR)assay,and the serum content of PAPP-A was determined by enzyme-linked immunosorbent assay(ELISA)assay.Results The levels of miR-15-5P in both the case group and the control group decreased with the increase of gestational age,while PAPP-A levels increased with the increase of gestational age,with statistical significance between different time points(P<0.05).The levels of miR-15-5P in the case group at 10,20,36 weeks of pregnancy and 1 week postpartum were lower than those in the control group,while the levels of miR-15-5P in the pregnancy induced hypertension group,mild preeclampsia group and severe preeclampsia group were successively decreased,with statistical significance between each group(P<0.05).The level of PAPP-A in pregnant women in case group was higher than that in control group at 10,20,36 weeks of pregnancy and 1 week postpartum.Meanwhile,the pregnancy induced hypertension group,mild preeclampsia group and severe preeclampsia group increased successively,and the difference between each group was statistically significant(P<0.05).Conclusions Dynamic monitoring of the changes of miR-15-5

关 键 词：妊娠期高血压 微小RNA-15-5P 妊娠相关血浆蛋白A 动态监测 诊断价值 

分 类 号：R714[医药卫生—妇产科学]