山楂炒焦前后组方大山楂丸的体内外化学成分分析  

Analysis of the chemical components in vivo and in vitro of Dashanzha Pill composed of raw and charred Hawthorn

作　　者：骆巧媚 范依霖 洪冉 孙小丽 刘翠 万军[1] LUO Qiao-mei;FAN Yi-lin;HONG Ran;SUN Xiao-li;LIU Cui;WAN Jun(Life Science&Engineering College of South-west Jiao-tong University,Chengdu 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《化学研究与应用》2023年第11期2577-2587,共11页Chemical Research and Application

基　　金：四川省科技厅基金项目(2020YJ0100)资助。

摘　　要：采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)技术,对山楂炒焦前后组方的生、焦山楂丸提取液的体内外化学成分进行分析和鉴定,对比研究其体内外效应物质差异。用ACQUITY UPLC BEH C_(18)色谱柱,以乙腈(A)-0.1%甲酸水溶液(B)为流动相梯度洗脱,在正、负离子模式下对山楂炒焦前后组方的生、焦山楂丸提取液、空白血浆及含药血清进行数据采集,结合对照品及专属数据库进行化学成分和血中移行成分的鉴定。从生山楂丸和焦山楂丸提取物中鉴别出化合物115个。生山楂丸入血成分30个,焦山楂丸入血成分34个,共有入血成分14个。生山楂丸特有入血成分16个,主要为黄酮类成分。焦山楂丸特有入血成分20个,以黄酮类成分和有机酸及其衍生物类成分为主。山楂丸的体内外物质基础以有机酸类和黄酮类为主,山楂炮制前后组成的生山楂丸和焦山楂丸间化学成分和血液移行成分有一定差异。To analyze and identify the chemical components of the extracts of Dashanzha Pill composed of crude and stir-baked haw-thorn by ultra-high performance liquid chromatography quadrupole/electrostatic field orbital trap high-resolution mass spectrometry(UPLC-Q-Orbitrap HRMS),and to compare the differences of their effective substances in vivo and in vitro.With ACQUITY UPLC BEH C_(18) chromatographic column,acetonitrile(a)-0.1%formic acid aqueous solution(b)was used as mobile phase gradient elu-tion,and the data of different Dashanzha Pill extracts and rat serum containing drugs were collected by ultra-performance liquid chromatography-high resolution mass spectrometry in positive and negative ion modes.And the chemical composition and blood mi-gration components were identified by combining with reference substances and proprietary database.A total of 115 compounds were identified in the extracts of Dashanzha Pill composed of raw and charred Hawthorn.There were 30 components in the serum given Shengshanzha Pill and 34 components in the serum given Jiaoshanzha Pill.There were 14 same blood components in Shengshanzha Pill and and Jiaoshanzha Pill.16 blood components in Shengshanzha Pill were different from Jiaoshanzha Pill,mainly flavonoids.And 20 blood components of Jiaoshanzha Pill were different from those of Shengshanzha Pill,mainly flavonoids,organic acids and their derivatives.Organic acids and flavonoids are the main substance bases of Hawthorn pill in vivo and in vitro.There are certain differences in chemical components and blood migration components between Shengshanzha Pill and Jiaoshanzha Pill before and af-ter Hawthorn processing.

关 键 词：大山楂丸 炒焦 超高效液相色谱-四级杆/ 静电场轨道阱高分辨质谱 化学成分 

分 类 号：O657.7[理学—分析化学]