Chinese Herb Formulae Inhibit the Proliferation of Human Colon Cancer SW480 Cells by Inducing Cell Apoptosis  

作　　者：Su Fu Shao-Lin Qin Wolf-Dieter Rausch 

机构地区：[1]Department for Internal Medicine of Traditional Chinese Medicine,Eye Hospital China Academy of Chinese Medical Sciences,Beijing,China [2]Department for Biomedical Sciences,Institute for Chemistry and Biochemistry,University of Veterinary Medicine Vienna,Vienna,Austria

出　　处：《World Journal of Traditional Chinese Medicine》2023年第3期348-354,共7页世界中医药杂志（英文）

基　　金：financially supported by the Austrian Federal Ministry of Education,Science,Research(GZ402.000/00015-WF/V/6/2016)granted to Dr.Wolf-Dieter Rausch.The project was named Sino-Austrian TCM research on lifestyle-related diseases:The potential of TCM for the treatment of colorectal cancer;the Eurasia-Pacific Uninet of the Austrian Agency for International Cooperation in Education and Research for the supports to PhD.Su FU in academic communication in Austria。

摘　　要：Objective:This study aimed to reveal the antitumor effects of Chinese herbal formulae and the underlying mechanisms in treating colorectal cancer,with a focus on developing traditional Chinese medicine(TCM)as a supplement and alternative therapeutic method for cancers.Materials and Methods:Human colon cancer SW480 cells were treated with three Chinese herbal formulae,Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction at different concentrations(50–600μg/mL)for 24,36,and 48 h,respectively.Cell viability was determined using the resazurin reduction assay,and cell survival rate was evaluated using a colony formation assay.After treatment with different concentrations(50–600μg/mL)of these three formulae for 48 h,the effects of the Chinese herbal formulae on cell apoptosis were investigated using Hoechst/propidium iodide(PI)staining.The positive PI-stained cells were investigated using an EnSpire multilabel plate reader and the positive Hoechst-stained cells were observed under a fluorescence microscope for morphological changes.Results:Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction inhibited SW480 cell proliferation in a dose-and time-dependent manner and induced cell apoptosis.Conclusion:Chinese herbal formulae with a special prescription form of TCM with antitumor effects bring a new perspective in line with the principles of TCM in cancer treatment.

关 键 词：Apoptosis cell proliferation Chinese herbal formulae colorectal cancer SW480 cells 

分 类 号：R285[医药卫生—中药学]