MALDI-TOF MS快速检测血流感染大肠杆菌对第3代头孢菌素的药敏研究  

Rapid Detection of the Third Generation Cephalosporins Sensitivity Test of Escherichia Coli Causing Blood Stream Infection by MALDI-TOF MS

作　　者：刘春林 黄德兵 吴清念 蔡甜 LIU Chunlin;HUANG Debing;WU Qingnian(Department of Clinical Laboratory,The Sixth Affiliated Hospital,School of Medicine,South China University of Technology,Guangdong 528200,China)

机构地区：[1]华南理工大学附属第六医院检验科,佛山528200

出　　处：《医学研究杂志》2023年第10期146-149,共4页Journal of Medical Research

基　　金：广东省医学科研基金资助项目(B2021428)。

摘　　要：目的评价基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight,MALDI-TOF MS)直接靶板微滴生长法(direct-on-target microdroplet growth assay,DOT-MGA)快速检测血流感染大肠杆菌对第3代头孢菌素药敏实验的价值。方法收集血流感染大肠杆菌50株作为实验菌株。采用菌液与不同浓度头孢噻肟在靶板上混合作为检测孔,不加抗菌药物的菌液作为生长对照孔,孵育4h后,根据DOT-MGA法鉴定靶板上的待测菌,所对应检测孔位代表头孢噻肟最低抑菌浓度,并以微量肉汤稀释法作为比较。结果微量肉汤稀释法和DOT-MGA法检测大肠杆菌对头孢噻肟最低抑菌浓度基本一致率为96%,分类一致率为94%,较大偏差为2%,次要偏差为4%,未发现重大偏差,均在临床可接受范围内。结论MALDI-TOF MS结合DOT-MGA法能够短时间内准确提供大肠杆菌对第3代头孢菌素的药敏信息,为临床早期精准管理血流感染患者提供依据。Objective To evaluate the value of Matrix-assisted laser desorption ionization-time of flight(MALDI-TOF MS) with direct-on-target microdroplet growth assay(DOT-MGA) method for rapid detection the third generation cephalosporins sensitivity test of Escherichia coli causing blood stream infection.Methods Fifty strains of blood stream infected causing by Escherichia coli were collected as experimental strains.The bacterial solution and cefotaxime of different concentrations were mixed on the target plate as the detection spots,and the bacterial solution without antibacteria was used as the growth control.After incubation for 4-hour,the bacteria on the target plate were identified according to DOT-MGA method.The corresponding detection spots position was used to represent the minimum inhibitory concentration(MIC) of cefotaxime,and the micro-broth dilution method was used for the comparison.ResultsThe basic agreement rate for the detection of MIC of Escherichia coli to cefotaxime by the micro-broth dilution method and the DOT-MGA method was 96%,the classification agreement rate was 94%,the larger deviation was 2%,the minor deviation was 4%,and no major deviation was found,which were all within the clinically acceptable range.Conclusion MALDI-TOF MS Combined with DOT-MGA can accurately provide the drug sensitivity information of Escherichia coli to the third generation cephalosporins in a short time,and provide evidence for the accurate management of patients with bloodstream infection in the early stage of clinical practice.

关 键 词：直接靶板微滴生长法 基质辅助激光解析电离飞行时间质谱 血流感染 第3代头孢菌素 最低抑菌浓度 

分 类 号：R44[医药卫生—诊断学]