高效液相色谱双柱串联技术用于维生素B3的高效分离测定  

High efficient separation and determination of vitamin B3 by high performance liquid chromatography with dual columns tandem technology

作　　者：司晓喜 杨怡 蔡晓丽 郑晗 王浩 刘志华 李振杰 许志刚[2] SI Xiaoxi;YANG Yi;CAI Xiaoli;ZHENG Han;WANG Hao;LIU Zhihua;LI Zhenjie;XU Zhigang(Yunnan Key Laboratory of Tobacco Chemistry/R&D Center of China Tobacco Yunnan Industrial Co.Ltd.,Kunming,Yunnan 650231,China;Faculty of Science,Kunming University of Science and Technology,Kunming,Yunnan 650500,China;Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)

机构地区：[1]云南烟草化学重点实验室/云南中烟工业有限责任公司技术中心,云南昆明650231 [2]昆明理工大学理学院,云南昆明650500 [3]昆明理工大学环境科学与工程学院,云南昆明650500

出　　处：《贵州师范大学学报（自然科学版）》2023年第6期85-89,共5页Journal of Guizhou Normal University：Natural Sciences

基　　金：云南省烟草化学重点实验室开放课题(2021539200340247);云南省科技厅科技计划项目“技术创新人才培养对象项目”(202105AD160006);云南中烟工业有限责任公司科技项目(JB2022HX01)。

摘　　要：建立了一种基于双色谱柱串联技术的高效液相色谱法,用于功能饮料以及药物中的维生素B3的测定。C18柱在前亲水相互作用色谱(HILIC)柱在后进行串联,流动相为乙腈-0.1%醋酸水溶液=10∶90 (V/V),柱温为30℃,流速为0.4 mL/min,紫外检测波长为262 nm。结果表明:在2个功能饮料样品中没有检测到维生素B3,在维生素B3粉末中检测到维生素B3的含量为0.15 mg/g。所建立方法的线性方程为y=1.85x-0.13,相关系数r为0.999 9,检出限为0.033 mg/L。加标回收实验的回收率在93.05%~119.35%之间,相对标准偏差在0.15%~4.50%之间。该方法能够对复杂基质样品中的维生素B3进行有效测定。A high performance liquid chromatography method based on dual columns tandem technology was established for the determination of vitamin B3 in functional beverages and drugs.The C 18 column was used in series before the HILIC column.The mobile phase consisted of acetonitrile-0.1%acetic acid solution(10∶90,V/V).The column temperature was 30℃,the flow rate was 0.4 mL/min,and the UV detection wavelength was 262 nm.The results showed that no vitamin B3 was found in the two functional beverages,but 0.15 mg/g vitamin B3 was detected in the vitamin B3 powder.The linear equation of the proposed method is y=1.85 x-0.13,the correlation coefficient r is 0.9999,and the detection limit is 0.033 mg/L.The recovery rate of spiked samples is between 93.05%~119.35%,and the relative standard deviation(RSD)is 0.15%~4.50%.This method can effectively determine vitamin B3 in complex matrix samples.

关 键 词：维生素B3 柱串联 C18柱 HILIC柱 高效液相色谱 

分 类 号：O657.7[理学—分析化学]