响应面优化混菌固态发酵对铁核桃饼粕饲料粗纤维含量的影响  

Effect of mixed solid-state fermentation on crude fiber content of Juglans sigllata cake by response surface optimization

作　　者：张天祎 谢晋奕 熊智[2] 顾瑞静 陶明晗 李娟 黄远航 韩龙 ZHANG Tianyi;XIE Jinyi;XIONG Zhi;GU Ruijing;TAO Minghan;LI Juan;HUANG Yuanhang;HAN Long(College of Life Science,Southwest Forestry University,Kunming,Yunnan Province 650224,China;College of Continuing Education,Southwest Forestry University,Kunming,Yunnan Province 650224,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]西南林业大学继续教育学院,云南昆明650224

出　　处：《中国饲料》2023年第22期340-346,共7页China Feed

基　　金：云南中烟工业重点项目(2023XL02);云南省重大科技专项(生物医药)(202002AA100007)。

摘　　要：本试验以铁核桃饼粕为原料,粗纤维含量为指标,探究料水比、总接种量、混菌接种比例、发酵温度、发酵时间对黑曲霉、产朊假丝酵母混菌固态发酵效果的影响。在单因素试验的基础上,通过响应面法(Box-Behnken)对发酵工艺进行优化,分析各因素及两因素间的交互作用对铁核桃饼粕粗纤维含量的影响。结果表明:最佳发酵工艺条件为:发酵温度28℃、发酵时间6 d、接种比例1:1,此条件下,发酵样品中粗纤维含量为32.07%,与发酵前相比降低了9.01%。The experiment used Juglans sigllata cake as raw material and crude fiber content as index,the effects of material-water ratio,total inoculation amount,inoculation ratio of mixed bacteria,fermentation temperature and fermentation time on solid-state fermentation of Aspergillus Niger and Candida utilis were explored.On the basis of single factor experiment,the fermentation process was optimized by response surface method(Box-Behnken),and the effects of each factor and the interaction between the two factors on the crude fiber content of Juglans sigllata cake were analyzed.The results showed that the optimum fermentation conditions were as follows:fermentation temperature 28℃,fermentation time 6 d and inoculation ratio 1:1.Under this condition,the crude fiber content in the fermentation sample was 32.07%,which was reduced by 9.01% compared with before fermentation.

关 键 词：铁核桃饼粕 固态发酵饲料 黑曲霉 产朊假丝酵母 粗纤维 

分 类 号：S816.6[农业科学—饲料科学]