卵泡大小、COCs形态和IGF-1、E2添加对驴卵母细胞体外成熟的影响  

Effects of Follicle Size,COCs Morphology and IGF-1 and E 2 Addition on Donkey Oocyte Maturation in Vitro

作　　者：李国超 朱新远 赵珊珊[1] 刘艺丹 董焕声[1] 潘庆杰[1] LI Guochao;ZHU Xinyuan;ZHAO Shanshan;LIU Yidan;DONG Huansheng;PAN Qingjie(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Rongcheng Animal Husbandry Bureau,Rongcheng 264300,China)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]荣成畜牧局,山东荣成264300

出　　处：《青岛农业大学学报（自然科学版）》2023年第4期257-264,共8页Journal of Qingdao Agricultural University(Natural Science)

基　　金：山东省动物种质资源保护与利用研究创新团队项目(0041619003);汗血马体细胞克隆研究与应用(660-2417014)。

摘　　要：为了研究卵泡直径大小、卵母细胞卵丘复合体(cumulus oocyte complexs,COCs)形态、季节因素对驴COCs以及胰岛素生长因子(insulin-like growth factor,IGF-1)与雌激素(estradiol,E_(2))、甘氨酸(glycine,Gly)对卵母细胞体外成熟的影响。试验以第一极体排出作为卵母细胞成熟的评判标准。结果表明,直径在1~3 cm的卵泡中,COCs的第一极体排出率为(45.33±3.34)%,极显著高于直径≤1 cm和直径≥3 cm的卵泡(33.92±4.34)%第一极体排出率(P<0.01);对不同类型COCs进行体外培养,扩展型(expanded,Ex)COCs组第一极体排出率为(54.26±2.41)%与紧凑型(compact,Cp)COCs组第一极体排出率(33.66±11.59)%具有显著差异(P<0.05);发情季节COCs第一极体排出率为(48.86±1.03)%,极显著高于非发情季节COCs的第一极体排出率(29.03±2.44)%(P<0.01)。此外,1μg/mL E_(2)能够极显著提高驴卵母细胞在非发情季节的成熟率(P<0.01),但是对其在发情季节的发育没有显著作用;在培养体系中添加200 ng/mL IGF-1,驴卵母细胞在发情季节和非发情季节的体外成熟都有极显著(P<0.01)的改善;在成熟液中添加6 mmol/mL Gly与对照组第一极体排出率无显著差异(P>0.05)。以上研究说明在驴卵母细胞体外成熟培养过程中,通过对卵泡直径大小、COCs类型和季节的选择以及IGF-1、E_(2)的添加,可获得较高的卵母细胞体外成熟率,有利于建立完善的驴卵母细胞体外成熟培养体系。In order to study the effects of follicle diameter,cumulus oocyte complexes(COCs)morphology,and seasonal factors on donkey COCs,insulin-like growth factor(IGF-1),and estrogen(E_(2)),Effect of glycine(Gly)on oocyte maturation in vitro.The test used the expulsion of the first polar body as the criterion for oocyte maturation.The results showed that in follicles with a diameter of 1 to 3 cm,the first polar body excretion rate of COCs was(45.33±3.34)%,which was extremely significantly higher than that of follicles with a diameter of≤1 cm and≥3 cm(33.92±4.34)%.The first polar body excretion rate(P<0.01);different types of COCs were cultured in vitro.The first polar body excretion rate of the expanded(Ex)COCs group was(54.26±2.41)%,while the first polar body excretion rate of the compact(compact,Cp)COCs group was(54.26±2.41)%.The first polar body excretion rate of COCs during the estrus season was(48.86±1.03)%,which was significantly different(P<0.05).(29.03±2.44)%(P<0.01).In addition,1μg/mL E_(2) could significantly increase the maturation rate of donkey oocytes in the non-estrus season(P<0.01),but it has no significant effect on its development in the estrus season;adding 200 ng/mL to the culture system IGF-1 significantly improved(P<0.01)the in vitro maturation of donkey oocytes in estrus and non-estrus seasons;adding 6 mmol/mL Gly to the maturation fluid was associated with the first polar body excretion rate in the control group.There was no significant difference(P>0.05).The above studies shows that during the in vitro maturation culture of donkey oocytes,a higher in vitro maturation rate of oocytes can be obtained by selecting the follicle diameter,COCs type and season,and adding IGF-1 and E_(2),which is conducive to the establishment of Complete donkey oocyte in vitro maturation culture system.

关 键 词：驴 卵母细胞 体外成熟 雌激素 卵泡 

分 类 号：S813.1[农业科学—畜牧学]