兰坪乌骨绵羊和普通绵羊血浆代谢组学比较分析  

Comparative Analysis of Plasma Metabonomics between Lanping Black-Boned Sheep and Conventional Sheep

作　　者：黄代涛 王波 陆颖 张锦标 和晓明 高振东 王楷 李雯 赵东祥 种玉晴 邓卫东[1] HUANG Daitao;WANG Bo;LU Ying;ZHANG Jinbiao;HE Xiaoming;GAO Zhendong;WANG Kai;LI Wen;ZHAO Dongxiang;CHONG Yuqing;DENG Weidong(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,昆明650201

出　　处：《动物营养学报》2023年第11期7368-7378,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(32160771);云南省院士(专家)工作站项目(202105AF150043);云南省重大科技专项计划(202202AE090005)。

摘　　要：本研究旨在对兰坪乌骨绵羊和普通绵羊在血浆代谢组学方面进行差异性比较。试验采用液相色谱串联质谱(LC-MS/MS)技术研究了12只兰坪乌骨绵羊和12只兰坪本地普通绵羊在血浆代谢组中不同代谢物特征。结果表明,共获得181种差异代谢物(P<0.05),其中阿苯达唑亚砜、阿苯达唑砜、4-羟基异亮氨酸等48种代谢物表达上调,麦考酚酸、异丙肾上腺素、18-羟基二十碳五烯酸等133种代谢物表达下调。代谢途径分析显示,差异代谢物富集于胆汁分泌、药物代谢-细胞色素P450、醛固酮调节的钠重吸收等9条通路,且在其中发现了6种标志代谢物(去氧胆酸、胆酸、石胆酸、鹅去氧胆酸、氢化可的松、雌三醇)参与调节兰坪乌骨绵羊的免疫、营养物质代谢、抗氧化能力等生物过程。通过研究结果显示出兰坪乌骨绵羊和普通绵羊血浆代谢组学上的差异,为深入了解兰坪乌骨绵羊和普通绵羊生长及抗逆性能的潜在代谢机制提供了理论依据。The present study aimed to investigate the plasma metabolomic difference between Lanping black-boned sheep and conventional sheep.A liquid chromatography-tandem mass spectrometry(LC-MS/MS)technique was employed to scrutinize the unique metabolic features in the plasma metabolome of 12 Lanping black-boned sheep and 12 local conventional sheep.The results showed as that a total of 181 differential metabolites were obtained(P<0.05),contained 48 up-regulated metabolites,including aminophosphonic acid sulfonamide,aminophosphonic acid sulfone and 4-hydroxyisoleucine,and contained 133 down-regulated metabolites,including ferulic acid,isoprenaline,and 18-hydroxyeicosapentaenoic acid.Pathway analysis revealed that these differential metabolites were enriched in nine distinctive pathways,such as bile secretion,drug metabolism-cytochrome P450,aldosterone-regulated sodium reabsorption,et al.Within this context,six emblematic signature metabolites(deoxycholic acid,cholic acid,lithocholic acid,goose deoxycholic acid,hydrocortisone,and estradiol)were discovered to intricately participate in modulating the immune,nutritional metabolism and antioxidative capacity of Lanping black-boned sheep.The findings from this research results show profound disparities in plasma metabolomics between Lanping black-boned sheep and conventional sheep,thus laying the groundwork for a more comprehensive comprehension of the underlying metabolic mechanisms governing the growth potential and resilience of Lanping black-boned sheep and conventional sheep.

关 键 词：兰坪乌骨绵羊 普通绵羊 代谢组学 

分 类 号：S826[农业科学—畜牧学]