哺乳仔猪轮状病毒和肠产毒性大肠杆菌混合感染病例的诊治  

Diagnosis and treatment of co-infection of enterotoxigenic Escherichia coli and rotavirus in suckling piglets

作　　者：祁松 张哲玮 朱晶 罗宇山 董德文 贝为成 QI Song;ZHANG Zhewei;ZHU Jing;LUO Yushan;DONG Dewen;BEI Weicheng(College of Veterinary Medicine and State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;Guangxi Xiangyi Biological Engineering Co.,Ltd.,Nanning 530000,China;Hubei Hongshan Laboratory,Wuhan 430070,China;The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院农业微生物学国家重点实验室,武汉430070 [2]广西翔仪生物工程有限公司,南宁530000 [3]湖北洪山实验室,武汉430070 [4]生猪健康养殖协同创新中心,武汉430070

出　　处：《黑龙江畜牧兽医》2023年第22期76-82,142,143,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家生猪产业技术体系项目(CARS-35);动物基因工程疫苗国家重点实验室开放课题(AGVSK-ZD-201805);畜禽病原微生物学湖北省重点实验室开放课题(KLPCAAB-2020-03)。

摘　　要：2022年8月中旬广西地区某规模化养猪场产房中7~15日龄哺乳仔猪发生严重腹泻和呕吐,甚至死亡,且死亡率较高。为查明病因和控制该病,试验首先根据临床症状和病理剖检结果进行初步判断;然后采集病料,分别对病毒性病原和细菌性病原进行进一步检测,并针对细菌性病原进行药敏试验;最后根据诊断结果进行有针对性的治疗。结果表明:腹泻哺乳仔猪小肠壁呈薄的半透明状,部分肠段呈灰黄色和灰黑色;肠壁充血,内容物为絮状的黄色或灰色液体;胃内充满黄色液体,可见少量凝乳块;初步判断为轮状病毒(RV)或猪德尔塔冠状病毒(PDCoV)和大肠杆菌的混合感染。进一步检测发现,病料中A群轮状病毒(RVA)呈阳性,猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和PDCoV呈阴性。从病料中分离到1株肠产毒性大肠杆菌(ETEC);该分离株对头孢曲松、庆大霉素和卡那霉素敏感,对氟苯尼考、多西环素和恩诺沙星中介,对阿莫西林、替米考星、泰万菌素和新霉素耐药。对产房腹泻仔猪按2~3 mL/头的剂量灌服10%庆大霉素进行治疗,每天1次,连续3 d,仔猪腹泻情况得到了有效控制。说明导致该场哺乳仔猪发生严重腹泻的主要原因为RV和ETEC混合感染,采取的治疗措施具有良好的效果。In mid-August 2022,suckling piglets aged 7 to 15 days in the delivery room of a large-scale pig farm in Guangxi area suffered from severe diarrhea and vomiting,and even died,with a high mortality rate.In order to identify the cause and control the disease,the test first made a preliminary judgment on the cause according to the clinical symptoms and pathological examination results.Then the samples were collected,the viral pathogens and bacterial pathogens were further detected,and the bacterial pathogens were tested for drug sensitivity.Finally,according to the diagnosis,targeted treatment was carried out.The results showed that the intestinal wall of diarrhea suckling piglets was thin and translucent,and some intestinal segments were grayish yellow and grayish black.The intestinal wall was congested,and the contents were flocculent yellow or gray fluid.The stomach was flled with yellow fluid,and a small amount of curd could be seen.The preliminary conclusion was RV or PDCoV and Escherichia coli mixed infection.Further tests showed that Group A rotavirus(RVA)was positive in the samples,while porcine epidemic diarrhea virus(PEDV),porcine transmissible gastroenteritis virus(TGEV)and porcine delta coronavirus(PDCoV)were negative.One strain of enterotoxigenic Escherichia coli(ETEC)was isolated from the samples.The isolate was sensitive to ceftriaxone,gentamicin and kanamycin,intermediated with parafenicol,doxycycline and enrofloxacin,and resistant to amoxicillin,temicosin,tevanistin and neomycin.Piglets with diarrhea in the delivery room were treated with 10%gentamicin at a dose of 2-3 mL/head;once a day for 3 consecutive days.After several days of treatment,the diarrhea of piglets was effectively controlled.The results showed that the main cause of severe diarrhea of suckling piglets in this farm was mixed infection of RV and ETEC,and the treatment measures had good effect.

关 键 词：哺乳仔猪 轮状病毒(RV) 肠产毒性大肠杆菌(ETEC) 腹泻 混合感染 诊治 

分 类 号：S858.28[农业科学—临床兽医学]