黄芩苷对尿酸钠诱导的鸡单核细胞炎性损伤的影响  

Effects of baicalin on sodium urate-induced inflammatory damage of chicken monocytes

作　　者：邓琴 余翔 何利琼 张维 邱银生 徐凌云[1] DENG Qin;YU Xiang;HE Liqiong;ZHANG Wei;QIU Yinsheng;XU Lingyun(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China;School of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生命科学与技术学院,武汉430023 [2]武汉轻工大学动物科学与营养工程学院,武汉430023

出　　处：《黑龙江畜牧兽医》2023年第22期123-129,共7页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了探讨黄芩苷(baicalin,BA)对尿酸钠诱导的鸡单核细胞炎性损伤的影响,试验从采集的鸡抗凝血中分离单核细胞,先检测不同浓度(5,10,20,40,80,160μmol/L)BA对正常鸡单核细胞活力的影响,选择对细胞活力无显著影响的BA浓度和培养时间,用2 mg/mL的尿酸钠晶体(monosodium urate,MSU)诱导细胞炎性反应,用不同浓度的BA干预细胞。试验分为空白对照组、MSU组(2 mg/mL)、MSU+不同浓度BA组,培养一定时间后用试剂盒检测细胞培养上清液中的一氧化氮(NO)浓度、乳酸脱氢酶(LDH)活力及白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)与肿瘤坏死因子-α(TNF-α)质量浓度。用荧光定量PCR方法检测细胞Toll样受体4(TLR4)、NOD样受体家族结构域3(NLRP3)、核因子-κB(NF-κB)、半胱氨酸蛋白酶-1(Caspase-1)、IL-1β、IL-18的mRNA相对表达量。结果表明:与空白对照组相比,MSU组NO浓度、LDH活力及IL-1β、IL-18、TNF-α质量浓度显著或极显著增加(P<0.05,或P<0.01,或P<0.001),TLR4/NF-κB和NLRP3信号通路基因mRNA相对表达量极显著增加(P<0.01)。与MSU组相比,MSU+40μmol/L BA组、MSU+80μmol/L BA组NO浓度显著或极显著下降(P<0.05或P<0.01);MSU+80μmol/L BA组LDH活力显著降低(P<0.05);MSU+20μmol/L BA组、MSU+40μmol/L BA组细胞上清液的IL-1β、IL-18、TNF-α质量浓度显著或极显著下降(P<0.05或P<0.01);除MSU+20μmol/L BA组IL-18 mRNA相对表达量未显著降低(P>0.05)外,MSU+10μmol/L BA组、MSU+20μmol/L BA组、MSU+40μmol/L BA组及MSU+80μmol/L BA组TLR4、NLRP3、NF-κB、Caspase-1、IL-1β、IL-18的mRNA相对表达量显著或极显著降低(P<0.05或P<0.01)。说明BA能够减轻MSU诱导的鸡单核细胞炎症损伤,其机制可能与下调炎症因子水平、抑制TLR4/NF-κB和NLRP3/Caspase-1信号通路相关基因表达有关。In order to investigate the effects of baicalin(BA)on sodium urate-induced inflammatory damage of chicken monocytes,in this experiment,monocytes were isolated from the collected chicken anticoagulant blood,and the effects of different concentrations(5,10,20,40,80,160μmol/L)of BA on normal chicken monocytes viability were first detected;and the BA concentration and culture time without significant effect on cell viability were selected for subsequent experiments.Cell inflammation was induced with 2 mg/mL of monosodium urate(MSU),and cells were intervened with different concentrations of BA;the experiment contained blank control group,MSU group(2 mg/mL)and MSU+BA group with different concentrations.After a certain period of culture,nitric oxide(NO)concentration,lactate dehydrogenase(LDH)activity,the mass concentrations of interleukin-1β(IL-1β),interleukin-18(IL-18)and tumor necrosis factor-α(TNF-α)in the cell culture supernatant were detected by the kits.The relative mRNA expression of Toll-like receptor 4(TLR4),NOD-like receptor family domain 3(NLRP3),nuclear factor-κB(NF-κB),cysteine protease-1(Caspase-1),IL-1βand IL-18 in cells were detected by real-time PCR.The results showed that the NO concentration,LDH activity,and mass concentrations of IL-1β,IL-18 and TNF-αin the model group increased significantly or very significantly(P<0.05,or P<0.01,or P<0.001)compared with the blank control group;the relative expression of TLR4/NF-κB and NLRP3 signaling pathway genes mRNA was very significant(P<0.01).Compared with the MSU group,The NO concentration decreased significantly or very significantly in the MSU+40μmol/L BA group and the MSU+80μmol/L BA group(P<0.05 or P<0.01);LDH viability decreased significantly in MSU+80μmol/L BA group(P<0.05);the mass concentrations of IL-1β,IL-18,TNF-αin the MSU+20μmol/L BA group and the MSU+40μmol/L BA group decreased significantly or very significantly(P<0.05 or P<0.01);except the relative expression of IL-18 mRNA in the MSU+20μmol/L BA group was not significantly re

关 键 词：黄芩苷 鸡单核细胞 尿酸钠 乳酸脱氢酶 TOLL样受体4 

分 类 号：S859.3[农业科学—临床兽医学]