高加索三叶草全长转录组测序及Dof转录因子家族分析  

Full-length Transcriptome Sequencing and Dof Transcription Factor Family Analysis of Caucasian Clover

作　　者：牟丹 赵啟军 刘玉英 李长慧[2] 谢久祥[2] MOU Dan;ZHAO Qi-jun;LIU Yu-ying;LI Chang-hui;XIE Jiu-xiang(State key laboratory of plateau ecology and agriculture,Qinghai University,Xining,Qinghai Province 810016,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai Province 810016,China)

机构地区：[1]青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016 [2]青海大学农牧学院,青海西宁810016

出　　处：《草地学报》2023年第11期3343-3354,共12页Acta Agrestia Sinica

基　　金：青海省“昆仑英才·高端创新创业人才”[青人才字(2023)1号];国家自然科学基金[32160334];青海大学2023年度青年科研基金[2023-QNY-5]资助。

摘　　要：为探究面临长时间低温胁迫的高加索三叶草(Trifolium ambiguum Bieb.)的转录特征,本研究通过PacBio Sequel平台进行全长转录组测序,获得70590条全长转录本(isoforms)。Isoforms的功能注释结果为:与红三叶的同源相似率最高,涉及信号转导机制、刺激响应、淀粉和蔗糖代谢通路、植物激素信号转导通路的Isoforms较多。Isoforms的结构分析结果为:预测到19693个SSR、2668条LncRNA、2788个AS和2917条TFs序列,TFs中的ERF,C3H,Dof等家族含序列数量较多。Dof转录因子家族的生物信息学分析结果为:42个Dof家族成员全部为亲水蛋白,大部分定位于细胞核;系统进化树分析将高加索三叶草与拟南芥Dof蛋白聚类为11个亚族;保守基序Motif1和Motif2出现的频率较高。以上发现可为下一步抗寒基因挖掘和功能验证提供数据支撑,并为解析高加索三叶草耐受长时间低温胁迫的生理生化机制奠定理论基础。In order to explore the transcriptional characteristics of Trifolium ambiguum Bieb.under long-term low temperature stress,the full-length transcriptome was sequenced by PacBio Sequel platform,and a total of 70590 full-length transcripts(Isoforms)were obtained.The functional annotation results of all Isoforms indicated the highest homologous similarity rate between caucasian clover and red clover existed,and the number of isoforms involving signal transduction mechanisms,stimulus response,starch and sucrose metabolism and plant hormone signal transduction pathways was the highest.Besides,the structural analysis results of all Isoforms showed that 19693 SSRs,2668 LncRNAs,2788 AS,and 2917 TFs were predicted and screened.The ERF,C3H,and Dof transcription factor families contained a larger number of isoforms.Moreover,the bioinformatics analysis of the Dof transcription factor family showed that all 42 Dof family members were hydrophilic proteins,most of which were located in the nucleus.The phylogenetic tree analysis clustered the Dof proteins of caucasian clover and Arabidopsis into 11 subfamilies.And the conserved Motif1 and Motif2 appeared more frequently.The above findings can provide a data support for the next step to cold resistance gene mining and functional verification,and lay a theoretical foundation for analyzing the physiological and biochemical mechanisms of caucasian clover tolerated to the long-term cold stress.

关 键 词：高加索三叶草 低温 全长转录组 DOF 

分 类 号：Q945.78[生物学—植物学]