河南省信阳地区家畜寄生蜱感染巴贝虫的分子流行病学调查  

Molecular epidemiological investigation of Babesia infection in ticks parasitized on domestic animals in Xinyang,Henan Province

作　　者：纪鹏慧 蒋甜甜 贺志权 王丹 岳思宁 李素华[1] 杨成运[1] 王昊[1] 张红卫[1] 周瑞敏[1] JI Penghui;JIANG Tiantian;HE Zhiquan;WANG Dan;YUE Sining;LI Suhua;YANG Chengyun;WANG Hao;ZHANG Hongwei;ZHOU Ruimin(Henan Center for Disease Control and Prevention,Henan Provincial Medical Key Laboratory of Parasitic Pathogen and Vector Provincial Key Laboratory for Infectious Disease Prevention and Control,Zhengzhou 450016,China)

机构地区：[1]河南省疾病预防控制中心,河南省寄生虫病原与媒介医学重点实验室,河南省传染病病原生物重点实验室,郑州450016

出　　处：《中国寄生虫学与寄生虫病杂志》2023年第5期567-572,共6页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20210134,LHGJ20220157)。

摘　　要：目的了解河南省信阳地区家畜寄生蜱感染巴贝虫的情况。方法2022年6—8月在河南省信阳市光山县和商城县采集家畜动物体表寄生蜱,采用形态学和PCR扩增蜱虫16S rDNA鉴定蜱虫种类。提取蜱虫基因组DNA,采用巢式PCR扩增巴贝虫18S rRNA基因。目的条带经测序后,进行BLAST比对分析,采用邻接法构建系统进化树,采用MEGA7.0软件进行同源性分析。结果共采集335只蜱,形态学和PCR扩增鉴定结果显示,长角血蜱49只、微小扇头蜱208只、褐黄血蜱1只、具环扇头蜱34只、刻点血蜱43只。PCR扩增结果显示,335份蜱虫样品中有2份样品扩增出巴贝虫400 bp大小的目的条带,蜱虫巴贝虫总阳性率为0.6%。BLAST比对分析结果显示,1份样品扩增出的18S rRNA序列与GenBank中来自美国(MK609547)、泰国(MG199181)、中国(KU204794)的田鼠巴贝虫序列相似性均达99.26%;另1份样品扩增出的18S rRNA序列与GenBank中来自美国(MH620203)、印度(MN161136)和中国(KP666166)的吉氏巴贝虫序列相似性均达100%。系统进化树分析结果显示,田鼠巴贝虫阳性样品与来自俄罗斯(KX987864)、中国(KU204794)、泰国(MG199179)等地的田鼠巴贝虫聚在同一分支上,同源性较高;吉氏巴贝虫阳性样品与来自中国(FJ769386)、美国(MH620203)和印度(KF606884)等地的吉氏巴贝虫聚在同一分支上,同源性较高。结论河南省信阳地区家畜寄生蜱存在田鼠巴贝虫和吉氏巴贝虫感染,可能对人和家畜有一定的致病风险。Objective To understand the infection of Babesia in ticks parasitized on domestic animals in Xin⁃yang,Henan Province.Methods Tick specimens were collected from domestic animals in Guangshan County and Shangcheng County,Xinyang City,Henan Province,from June to August 2022.The tick species were identified by morphology and 16S rDNA.Genomic DNA was extracted from tick specimens,and nested⁃PCR was performed to am⁃plify 18S rRNA gene sequence of Babesia.The PCR products were sequenced and aligned by BLAST,and phyloge⁃netic trees were constructed by the neighbor⁃joining method.Using MEGA7.0 software for homology analysis.Results A total of 335 ticks were collected,including the species of 49 Haemaphysalis longicornis,208 Rhipicephalus micro⁃plus,1 H.flava,34 R.annulatus,and 43 H.punctataa,identified by morphology and PCR amplification.The PCR amplification results showed that 2 out of 335 tick samples were amplified target bands of 400 bp,and the overall positive rate was 0.6%.The BLAST comparison analysis results showed that the 18S rRNA sequence amplified from one sample had a 99.26%similarity with the sequences of B.microti from the United States(MK609547),Thailand(MG199181),and China(KU204794)in GenBank.The 18S rRNA sequence amplified from another sample shares 100%similarity with the sequences of B.gibsoni from the United States(MH620203),India(MN161136),and China(KP666166)in GenBank.The phylogenetic tree analysis revealed that the B.microti positive sample was clustered on the same branch with B.microti from Russia(KX987864),China(KU204794)and Thailand(MG199179),showing a high homology.The B.gibsoni⁃positive samples were clustered on the same branch with B.gibsoni from China(FJ769386),USA(MH620203)and India(KF606884),showing a close relation.Conclusion The infections of B.mi⁃croti and B.gibsoni were detected in ticks parasitized on domestic animals in Xinyang area of Henan Province,which may render risk of disease transmission to humans and domestic animals.

关 键 词：蜱 巴贝虫 家畜 分子流行病学 信阳 河南省 

分 类 号：R531.5[医药卫生—内科学]