胚胎植入前单基因遗传学检测患者胚胎整倍体率的影响因素分析  

Analysis of influencing factors on embryo euploidy rate in patients with preimplantation genetic testing for monogenic diseases

作　　者：马聪聪 龙晓宇[1] 闫丽盈[1] 朱小辉[1] 陈立雪[1] 李蓉[1] 王颖[1] 乔杰[1] MA Congcong;LONG Xiaoyu;YAN Liying;ZHU Xiaohui;CHEN Lixue;LI Rong;WANG Ying;QIAO Jie(Reproductive Medicine Center,Peking University Third Hospital,Beijing 100191,China)

机构地区：[1]北京大学第三医院妇产科,生殖医学中心,女性生育力促进全国重点实验室,国家妇产疾病临床医学研究中心,辅助生殖教育部重点实验室,生殖内分泌与辅助生殖技术北京市重点实验室,北京100191

出　　处：《中国妇产科临床杂志》2023年第6期564-567,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：国家自然科学基金(81701407);国家重点研发计划(2022YFC2702901,2022YFC2703004)。

摘　　要：目的探讨胚胎植入前单基因遗传学检测(preimplantation genetic testing for monogenic diseases,PGT-M)患者中胚胎整倍体率的影响因素。方法回顾性纳入北京大学第三医院生殖医学中心行PGT-M的患者599例,按女方年龄分为3个亚组:<35岁、35~37岁和38~40岁。采用Spearman相关分析患者各临床特征与胚胎整倍体率的相关性,并采用广义线性回归分析两个或以上临床特征对胚胎整倍体率的影响。结果Spearman相关分析结果显示,在年龄<35岁组,胚胎整倍体率与获卵数呈负相关(r=-0.080,P=0.048);38~40岁组中胚胎整倍体率与获卵数呈正相关(r=0.279,P=0.011)。线性回归分析结果显示,在调整年龄因素后,<35岁组胚胎整倍体率与获卵数的相关性无统计学意义(P=0.865);而38~40岁亚组中胚胎整倍体率与获卵数呈正相关(β=0.234,P=0.037)。结论在PGT-M患者中未发现胚胎整倍体率的危险因素。在高龄(38~40岁)患者中,获卵数增加或可提高胚胎整倍体率。Objective To assess the influencing factors of embryo euploidy rate in patients undergoing preimplantation genetic testing for monogenic diseases(PGT-M).Methods Patients who underwent PGT-M at the Reproductive Medicine Center of Peking University Third Hospital were retrospectively included.Three subgroups were divided according to maternal age:<35,35~37 and 38~40 years old.The correlation between patient clinical characteristics and embryo euploidy rate was investigated by means of Spearman correlation analysis and linear regression when appropriate.Results Spearman correlation analysis showed that in the patients aged<35 years,the euploid rate of embryos was negatively correlated with the number of retrieved oocytes(r=-0.080,P=0.048);In patients aged 38~40 years,the euploid rate of embryos was positively correlated with the number of retrieved oocytes(r=0.279,P=0.011).Linear regression analysis showed that,after adjusting for maternal age,there was no significant correlation between the euploid rate of embryos and the number of retrieved oocytes in patients aged<35 years(P=0.865);The euploid rate of embryos was still positively correlated with the number of oocytes(β=0.234,P=0.037).Conclusion No risk factors for embryonic euploidy rate were found in PGT-M patients.In women with advanced age(38~40 years old),an increased number of retrieved oocytes may increase the embryo euploidy rate.

关 键 词：胚胎整倍体率 胚胎植入前单基因遗传学检测 年龄 获卵数 

分 类 号：R714.8[医药卫生—妇产科学]