阿托伐他汀联合臻通集胶囊治疗高脂血症模型大鼠的代谢组学研究  

Metabonomics study of atorvastatin combined with Zhentongji capsule on hyperlipidemia model of rats

作　　者：侯红平 罗倩 王彩霞 魏晓露 王丽芳 赵晓晓 陈腾飞 宋玲 高云航 李丽丽 彭博 张广平 Hou Hongping;Luo Qian;Wang Caixia;Wei Xiaolu;Wang Lifang;Zhao Xiaoxiao;Chen Tengfei;Song Ling;Gao Yunhang;Li Lili;Peng Bo;Zhang Guangping(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Hebei Yuzhilin Biotech Co.,Ltd.,Shijiazhuang 050000,China)

机构地区：[1]中国中医科科学院中药研究所,北京100700 [2]河北御芝林生物科技有限公司,石家庄050000

出　　处：《广西医科大学学报》2023年第10期1682-1691,共10页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82104502);中央级公益性科研院所基本科研业务费专项基金资助项目(No.ZXKT23006,No.ZZ13-YQ-060,No.ZXKT21020)。

摘　　要：目的:基于代谢组学探究阿托伐他汀联合臻通集胶囊治疗高脂血症模型大鼠的疗效和作用机制。方法:SD大鼠随机取6只作为对照组,正常饮食、饮水。其余动物均给予高脂饲料,连续4周后,甘油三酯(TG)和胆固醇(CHO)明显升高视为模型成功;根据CHO水平进行分组,分为模型组、西药组(阿托伐他汀钙片,0.9 mg/kg)、中药组(臻通集胶囊,0.216 g/kg)、中西医联合组(二者联用)。连续给药8周后,检测各组动物血清中的血脂水平,肝脏组织用于高分辨非靶代谢组学的研究。结果:模型组TG、CHO、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)水平较对照组显著升高(P<0.05);与模型组相比,西药组、中药组和中西医联合组血脂水平显著降低(P<0.05);与对照组相比,模型组共检测出138个差异代谢物;与模型组相比,西药组共检测出64个差异代谢物,中药组共检测出123个差异代谢物,中西医联合组共检出106个差异代谢物;中药组与西药组相比,共检出101个差异代谢物;经相关性分析和KEGG通路分析,主要涉及衰老、脂质代谢、氨基酸代谢、膜转运等通路。结论:阿托伐他汀联合臻通集胶囊能够有效地改善高血脂大鼠的血脂水平,代谢组学分析示其可能通过调节c GMP-PKG信号通路相关因子的表达,达到抑制肝脏细胞坏死的目的。Objective:To explore the therapeutic effect and mechanism of atorvastatin combined with Zhentongji capsule on hyperlipidemia model rats through metabonomics.Methods:Six SD rats were randomly selected as the control group,and they were given normal diet and water.The other animals were given high-fat feed,and after 4 weeks,triglyceride(TG)and cholesterol(CHO)increased significantly,which was regarded as the success of the model.According to CHO index,they were divided into model group,western medicine group(atorvastatin calcium tablet,0.9 mg/kg),traditional Chinese medicine group(Zhentongji capsule,0.216 g/kg)and integrated traditional Chinese and western medicine group(both combined).After 8 weeks of continuous administration,the serum lipid levels of animals in each group were detected,and the liver tissue was used for high-resolution untargeted metabolomics research.Results:The levels of TG,CHO,high density lipoprotein(HDL)and low density lipoprotein(LDL)in the model group were significantly higher than those in the control group(P<0.05).Compared with the model group,the blood lipid levels in the western medicine group,the traditional Chinese medicine group and the integrated traditional Chinese and western medicine group significantly reduced(P<0.05).Compared with the control group,138 different metabolites were detected in the model group.Compared with the model group,64 different metabolites were detected in the western medicine group,123 in the traditional Chinese medicine group and 106 in the integrated traditional Chinese and western medicine group.Compared with the western medicine group,101 different metabolites were detected in the traditional Chinese medicine group.Through correlation analysis and KEGG pathway analysis,it was mainly involved aging,lipid metabolism,amino acid metabolism,membrane transport and other pathways.Conclusion:Atorvastatin combined with Zhentongji capsule can effectively improve the blood lipid levels of hyperlipidemia rats.Metabolomics analysis shows that it may achieve the pu

关 键 词：阿托伐他汀 臻通集 高脂血症 代谢组学 中西医联合 

分 类 号：R285.5[医药卫生—中药学]