青蒿琥酯对高糖环境下大鼠下颌下腺上皮细胞凋亡的影响  

Effect of artesunate on apoptosis of rat submandibular gland epithelial cells in high glucose environment

作　　者：赵丹妮 王玉琛 农晓琳[1] Zhao Danni;Wang Yuchen;Nong Xiaolin(College&Hospital of Stomatology,Guangxi Medical University,Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Guangxi Clinical Research Center for Craniofacial Deformity,Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases,Key Laboratory of Research and Application of Stomatological Equipment,Nanning 530021,China)

机构地区：[1]广西医科大学口腔医学院/附属口腔医院,广西口腔颌面修复与重建研究重点实验室,广西颅颌面畸形临床医学研究中心,广西壮族自治区卫生健康委员会口腔感染性疾病防治重点实验室,广西高校口腔医学装备研发与应用重点实验室,南宁530021

出　　处：《广西医科大学学报》2023年第9期1440-1447,共8页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82160187)。

摘　　要：目的:研究青蒿琥酯(ART)对体外高糖环境下大鼠下颌下腺上皮细胞凋亡的影响。方法:通过体外培养、纯化并鉴定大鼠下颌下腺上皮细胞。使用CCK-8和western blotting检测不同浓度葡萄糖对大鼠下颌下腺上皮细胞增殖和功能的影响。使用含50 mmol/L葡萄糖的培养基模拟体外高糖环境,将细胞分为正常对照组(Con组)、高糖模型组(HG组)和不同浓度ART干预组(HG+5μmol/L ART组、HG+10μmol/L ART组、HG+20μmol/L ART组、HG+40μmol/L ART组)。分别采用实时荧光定量PCR(RT-qPCR)法和western blotting法检测细胞水通道蛋白5(AQP5)、Bax、Bcl-2和Caspase-3基因和蛋白表达。结果:随着高糖环境中葡萄糖浓度的增加,大鼠下颌下腺上皮细胞抑制率升高,AQP5蛋白表达水平降低(P<0.05)。与Con组相比,HG组细胞AQP5蛋白、Bcl-2基因和蛋白表达水平明显降低,Bax、Caspase-3基因和蛋白表达水平明显升高(P<0.05);与HG组相比,不同浓度ART干预组Bcl-2基因和蛋白表达水平及AQP5蛋白表达升高,Bax、Caspase-3基因和蛋白表达水平降低(P<0.05)。结论:ART对高糖环境下大鼠下颌下腺上皮细胞具有保护作用,其机制可能与抑制细胞凋亡有关。Objective:To investigate the effect of artesunate(ART)on apoptosis of rat submandibular gland epithelial cells in high glucose environment in vitro.Methods:Rat submandibular gland epithelial cells were cultured,purified and identified in vitro.The CCK-8 assay and western blotting were used to detect the effects of different concentrations of glucose on the proliferation and function of rat submandibular gland epithelial cells.The medium containing 50 mmol/L glucose was used to simulate the high glucose environment in vitro.The cells were divided into normal control group(Con group),high glucose model group(HG group)and different concentrations of ART intervention groups(HG+5μmol/L ART group,HG+10μmol/L ART group,HG+20μmol/L ART group,HG+40μmol/L ART group).The expression levels of aquaporin 5(AQP5),Bcl-2 associated X protein(Bax),B cell lymphoma/leukemia 2 protein(Bcl-2)and Caspase-3 were detected by real-time fluorescence quantitative PCR(RT-qPCR)and western blotting.Results:With the increase of glucose concentration in high glucose environment,the inhibition rate of rat submandibular gland epithelial cells increased and the protein expression level of AQP5 decreased(P<0.05).Compared with the Con group,the expression levels of AQP5 protein,Bcl-2 gene and protein in the HG group significantly decreased,while the expression levels of Bax,Caspase-3 gene and protein significantly increased(P<0.05).Compared with the HG group,the expression levels of Bcl-2 gene and protein and AQP5 protein in different concentrations of ART intervention groups increased,while the expression levels of Bax,and Caspase-3 gene and protein decreased(P<0.05).Conclusion:ART has a protective effect on rat submandibular gland epithelial cells in high glucose environment,and its mechanism may be related to the inhibition of apoptosis.

关 键 词：青蒿琥酯 下颌下腺上皮细胞 高糖环境 

分 类 号：R963[医药卫生—微生物与生化药学]