珍珠李炭疽菌对三种杀菌剂的敏感性测定  

Sensitivity Determination to Three Fungicides against Pearl Plum Anthracnose

作　　者：桂卿 黄荣 孙文秀[2] 唐利华[1] 黄穗萍[1] 陈小林[1] 郭堂勋[1] 莫贱友[1] 李其利[1] GUI Qing;HUANG Rong;SUN Wen-xiu;TANG Li-hua;HUANG Sui-ping;CHEN Xiao-lin;GUO Tang-xun;MO Jian-you;LI Qi-li(Inst.of Plant Protect.,Guangxi Academy of Agric.Sci./Key Lab.of Green Prevent.&Ctrl.on Fruits and Veget.in S.China,Minist.of Agric.&Rural Affairs/Guangxi Key Lab.of Biol.for Crop Diseases&Insect Pests,Nanning 530007;Coll.of Life Sci.,Yangtze Uni.,Jingzhou 434025)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所、农业农村部华南果蔬绿色防控重点实验室、广西作物病虫害生物学重点实验室,广西南宁530007 [2]长江大学生命科学学院,湖北荆州434025

出　　处：《微生物学杂志》2023年第5期71-80,共10页Journal of Microbiology

基　　金：广西重点研发计划项目(桂科AB20159041)。

摘　　要：由胶孢炭疽菌复合种(Colletotrichum gloeosporioides species complex)引起的炭疽病是珍珠李(Prunus salicina Lind1)产业生产上的主要病害,为明确珍珠李炭疽菌对吡唑醚菌酯、苯醚甲环唑和咪鲜胺的敏感性,比较不同地理来源及不同种炭疽菌对三种药剂敏感性差异。采用菌丝生长速率法分别测定三种杀菌剂对采自不同地区26株珍珠李炭疽菌的药剂敏感性。结果表明,供试的26株珍珠李炭疽菌对咪鲜胺的敏感性最高,EC_(50)值介于0.0108~0.6452μg/mL之间,平均值为(0.1912±0.03)μg/mL;苯醚甲环唑次之,EC_(50)值介于0.1658~2.1680μg/mL之间,平均值为(0.7258±0.0880)μg/mL;吡唑醚菌酯最低,EC_(50)值介于0.0583~84.5660μg/mL之间,平均值为(6.2763±3.5255)μg/mL。供试的26株珍珠李炭疽菌整体对以上三种杀菌剂敏感,且对咪鲜胺的敏感性最高,苯醚甲环唑次之,对吡唑醚菌酯的敏感性最低。本研究结果可为珍珠李炭疽病的化学防治提供科学参考。In order to determine the sensitivity of Colletotrichum gloeosporioides species complex collected from Longtan pearl plum orchard to three commonly used fungicides,pyraclostrobin,difenoconazole and prochloraz,and compare the sensitivity differences between different geographical origin and different species of Colletotrichum isolates to three fungicides,the sensitivity of 26 isolates was determined with mycelial growth rate method.The results indicated that 26 isolates were most sensitive to prochloraz,the 50%effective concentration(EC_(50))value was between 0.0108-0.6452μg/mL,and mean EC_(50) value was(0.1912±0.03)μg/mL;difenoconazole was the second,the EC_(50)value was between 0.1658-2.1680μg/mL,and mean EC_(50) value was(0.7258±0.088)μg/mL;pyraclostrobin was the lowest,the EC_(50)value was between 0.0583-84.5660μg/mL,and mean EC_(50) value was(6.2763±3.5255)μg/mL.26 isolates of Colletotrichum species were generally sensitive to above three fungicides,and the sensitivity to prochloraz was the highest,followed by difenoconazole,and the sensitivity to pyraclostrobin was the lowest.The results of this study provided references for the chemical control of Longtan pearl plum leaf blight.

关 键 词：珍珠李 炭疽病 胶孢炭疽菌复合种 吡唑醚菌酯 苯醚甲环唑 咪鲜胺 敏感性 

分 类 号：Q93-33[生物学—微生物学]