基于HPLC-QAMS/GC法联合多元统计分析及加权TOPSIS法评价广藿香质量  

Quality evaluation of Pogostemonis Herba based on HPLC-QAMS/GC combined with multivariate statistical analysis and weighted TOPSIS method

作　　者：吴学辉[1] 程心玲[1] 潘艳琳[1] 张晓斌 肖钦[1] WU Xue-hui;CHENG Xin-ling;PAN Yan-lin;ZHANG Xiao-bin;XIAO Qin(Department of Pharmacy,The People’s Hospital Affiliated to Fujian University of TCM,Fuzhou 350004,China;Fujian Institute for Food and Drug Quality Control,Fuzhou 350001,China)

机构地区：[1]福建中医药大学附属人民医院药学部,福建福州350004 [2]福建省食品药品质量检验研究院,福建福州350001

出　　处：《中成药》2023年第11期3670-3677,共8页Chinese Traditional Patent Medicine

基　　金：福建省科技厅引导性项目(2018Y0042)。

摘　　要：目的基于HPLC-QAMS/GC法多组分定量,建立多元统计分析及加权TOPSIS模型,对不同产地广藿香进行综合质量评价。方法HPLC-QAMS法同时测定新西兰牡荆苷、紫葳新苷Ⅱ、毛蕊花糖苷、列当苷、异毛蕊花糖苷、鼠李素、芹菜素-7-O-β-D-葡萄糖醛酸苷、藿香黄酮醇、雷杜辛黄酮醇、广藿香酮的含量。GC法测定百秋李醇含量。主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)筛选影响广藿香化学成分差异的标志性成分。加权TOPSIS模型对广藿香整体质量进行分析和评价。结果10种成分在各自范围内线性关系良好(r≥0.9991),平均加样回收率96.85%~100.20%,RSD 0.67%~1.64%。毛蕊花糖苷、百秋李醇、广藿香酮、列当苷是影响广藿香产品质量的主要潜在标志物。18批不同产地广藿香加权TOPSIS法质量评价贴近度(D_(n))分别为0.3821、0.5129、0.5125、0.5358、0.5357、0.3588、0.5365、0.5483、0.2063、0.1782、0.1426、0.1174、0.7169、0.7495、0.6645、0.7381、0.6234、0.5821,表明不同产地广藿香药材存在质量差异,广东产的广藿香质量较优。结论该方法操作便捷、结果准确,可用于广藿香的综合质量评价。AIM To establish the multivariate statistical analysis and weighted TOPSIS model for the comprehensive quality assessment of Pogostemonis Herba from different habitats based on HPLC-QAMS/GC multi-component quantification.METHODS The content determinations of viceninⅡ,campneosideⅡ,acteoside,crenatoside,isoacteoside,rhamnetin,apigenin-7-O-β-D-glucuronic acid glycoside,pachypodol,retusin,pogostone were conducted by HPLC-QAMS method;and patchouli alcohol by GC method.Pogostemonis Herba had its marker components screened by principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA);and its overall quality analyzed and assessed by the weighted TOPSIS model.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.9991),whose average recoveries were 96.85%-100.20%with the RSDs of 0.67%-1.64%.Acteoside,patchouli alcohol,pogostone and crenatoside might be the main potential markers affecting the quality of Pogostemonis Herba.The values of quality evaluation closeness(D_(n))of 18 batches of Pogostemonis Herba from different habitats by weighted TOPSIS method were 0.3821,0.5129,0.5125,0.5358,0.5357,0.3588,0.5365,0.5483,0.2063,0.1782,0.1426,0.1174,0.7169,0.7495,0.6645,0.7381,0.6234 and 0.5821,respectively,suggesting the quality differences due to different habitats,and a better quality seen in those from Guangdong.CONCLUSION This method is easy to operate and accurate in result,and can be used for the comprehensive quality evaluation of Pogostemonis Herba.

关 键 词：广藿香 主成分分析(PCA) 正交偏最小二乘法-判别分析(OPLS-DA) 优劣解距离法(TOPSIS) 质量评价 HPLC-QAMS/GC 

分 类 号：R284[医药卫生—中药学]