聚(1-乙烯基-2-吡咯烷酮)包覆明胶纳米颗粒的制备及其抗生物淤积性能研究  

Fabrication of Poly(1-Vinyl-2-Pyrrolidone)Encapsulated Gelatin Nanoparticles and Investigation of Their Anti-Biofouling Properties

作　　者：贾正勋 沈畯 陈栋 董学林 方雅琴 Jia Zhengxun;Shen Jun;Chen Dong;Dong Xuelin;Fang Yaqin(Hubei Geological Research Laboratory,Wuhan 430034,China;Hubei Provincial Institute of Spatial Planning and Research,Wuhan 430000,China;Hunan Changling Petrochemical Technology Development Co.,Ltd.,Yueyang 414000,China)

机构地区：[1]湖北省地质实验测试中心,湖北武汉430034 [2]湖北省空间规划研究院,湖北武汉430000 [3]湖南长岭石化科技开发有限公司,湖南岳阳414000

出　　处：《山东化工》2023年第19期42-45,共4页Shandong Chemical Industry

摘　　要：通过两步脱溶剂法制备得到了具有均匀尺寸分布的明胶纳米颗粒(GNPs)。以GNPs为模板利用原位自由基聚合方法在其表面包覆聚(1-乙烯基-2-吡咯烷酮)(PVP)的交联聚合物外壳,得到了具有明确核-壳结构的复合纳米颗粒GNPs@PVP。通过动态光散射(DLS)、透射电子显微镜(TEM)、傅立叶变换红外光谱(FTIR)等对该体系的尺寸分布、表面电荷、微观形貌和结构等进行了表征,并通过小鼠血清蛋白吸附实验评价了体系的抗生物淤积效果。结果表明,相比于包覆前的明胶纳米颗粒,GNPs@PVP的粒径有了明显增加,而表面电荷则由负电性转变为趋近于电中性。在m(GNPs)∶m(NVP)=1∶10的条件下,纳米微粒成膜后的非特异性蛋白质吸附率从21.1%±1.7%降低至1.2%±0.2%,表现出了良好的抗生物淤积效果。Gelatin nanoparticles(GNPs)with uniform size distribution were prepared by two-step desolvation method.The obtained GNPs as templates were then encapsulated with poly(1-vinyl-2-pyrrolidone)(PVP)polymer shell via in situ free radical polymerization to fabricate core-shell nanoparticles [e-mail] distribution,surface charge,microscopic morphology and complex structure of GNPs@PVP were characterized by dynamic light scattering(DLS),transmission electron microscope(TEM)and Fourier transform infrared spectroscopy(FTIR)respectively.The anti-biofouling property of GNPs@PVP was evaluated by protein adsorption assay using mice serum as model.It was found that the size of GNPs@PVP increased obviously compared with GNPs,while the surface charge shifted from electron-negative to approximately neutral state.Under the condition that m(GNPs)∶m(NVP)is 1∶10,GNPs@PVP showed significantly improved anti-biofouling capability that the non-specific protein adsorption ratio(NSPAR)decreased from 21.1%±1.7%to 1.2%±0.2%.

关 键 词：原位自由基聚合 1-乙烯基-2-吡咯烷酮 明胶纳米颗粒 核-壳结构 抗生物淤积 

分 类 号：TQ314[化学工程—高聚物工业] TB383[一般工业技术—材料科学与工程]