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作 者:孙健智 冉文青 冯立超[1] 崔娟 范文忠[1] 潘业兴[1] 赵丽娜[1] SUN Jianzhi;RAN Wenqing;FENG Lichao;CUI Juan;FAN Wenzhong;PAN Yexing;ZHAO Lina(Jilin Agricultural and Technical College,Jilin 132101,Jilin,China)
出 处:《农药》2023年第11期828-832,共5页Agrochemicals
基 金:吉林农业科技学院博士启动基金项目(吉农院合字[2021]第5020号),数字农业学科支持。
摘 要:[目的]明确草莓黑斑病病原菌种类,为其防治提供理论依据。[方法]根据形态学和rDNA-ITS序列比对鉴定,通过柯赫氏法则验证,确定病原菌的分类地位,并测定了病原菌对10种药剂的敏感性。[结果]草莓黑斑病病原菌为细极链格孢(Alternaria tenuissima),10%苯醚甲环唑WG、25%丙环唑EC和30%啶氧菌酯SC对菌丝的敏感性较高,EC50值分别为14.43、20.61、37.18 mg/L;其对孢子的敏感性亦较高,EC50值分别为132.10、205.83、120.84 mg/L。田间试验表明10%苯醚甲环唑WG、30%啶氧菌酯SC和25%丙环唑EC可以有效地防治草莓黑斑病,防效达75~97%。[结论]明确了草莓黑斑病病原菌种类,筛选出3种药剂可以有效地防治草莓黑斑病发生。[Aims]This study aims to identify the pathogen causing strawberry black spot and to provide a scientific basis for control of the black spot.[Methods]Based on Koch′s postulates,morphological characters and rDNA-ITS sequences analysis,taxonomic status of pathogen was determined,and its susceptibility to 10 fungicides were tested.[Results]The pathogen of strawberry black spot was Alternaria tenuissima.Difenoconazole 10%WG,propiconazol 25%EC,picoxystrobin 30%SC had a high inhibition activity on the mycelia growth with EC50 values of 14.43,20.61 and 37.18 mg/L respectively,and the fungicides had strong inhibitory effect on spore germination with EC50 values of 132.10,205.83 and 120.84 mg/L respectively.The field efficacy trials showed that the black spot were effectively controlled by difenoconazole 10%WG,propiconazol 25%EC,picoxystrobin 30%SC,with the control efficacy ranging from 75%to 97%.[Conclusions]The pathogen of strawberry black spot is identified as A.tenuissima.Three fungicides can effectively control the black spot caused by A.tenuissima.
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