肝肠胞虫对凡纳滨对虾肠道和肝胰腺菌群的影响  

SHRIMP HEPATIC ENTEROCYTES ON INTESTINAL AND HEPATOPANCREAS FLORA OF LITOPENAEUS VANNAMEI

作　　者：李舢舢 赵晨曦 吴波[1] 郑侠飞 戴文芳 林志华[1] 柳敏海 LI Shan-Shan;ZHAO Chen-Xi;WU Bo;ZHENG Xia-Fei;DAI Wen-Fang;LIN Zhi-Hua;LIU Min-Hai(Key Laboratory of Research on Efficient Utilization Technology of Aquatic Germplasm Resources,Ninghai Institute of Marine Biological Seed Industry,Zhejiang Wanli College,Ningbo 315000,China)

机构地区：[1]浙江万里学院宁海海洋生物种业研究院,浙江省水产种质资源高效利用技术研究重点实验室,宁波315000

出　　处：《水生生物学报》2024年第2期315-323,共9页Acta Hydrobiologica Sinica

基　　金：浙江省农业新品种选育重大科技专项(2021C02069-5-4);浙江省“三农九方”科技协作计划“揭榜挂帅”项目(2023SNJF064);宁波市重点研发计划暨“揭榜挂帅”项目(2023Z113);浙江省公益技术应用研究计划(LGN21C190011);国家现代农业产业技术体系资助。

摘　　要：利用16S rRNA高通量测序对比研究了健康对虾和感染肝肠胞虫的发病对虾肠道及肝胰腺菌群的组成、多样性、微生物介导的功能和种间互作间的差异性。结果表明,健康对虾的肠道和肝胰腺细菌群落的多样性高于发病对虾。在属水平上,健康对虾和发病对虾的肠道及肝胰腺中的优势细菌组成及丰度存在明显不同,其中健康对虾肠道中的优势菌属主要为埃希杆菌-志贺菌属(Escherichia-Shigella)和海绵菌属(Spongiimonas),而发病对虾肠道中的优势菌属则为希瓦式菌属(Shewanella)、巨球型菌属(Megasphaera)和克雷伯氏菌属(Klebsiella);健康对虾肝胰腺中的优势菌属主要为假单胞菌属(Pseudomonas)和汉氏盐单胞菌(Halomonas),而发病对虾肝胰腺中的优势菌属则为贪铜菌属(Cupriavidus)。Tax4Fun2预测显示,健康对虾肠道和肝胰腺菌群的主要功能分别与发病对虾肠道和肝胰腺菌群的主要功能有明显差异。研究有助于了解肝肠胞虫对凡纳滨对虾肠道及肝胰腺细菌群落的影响,并为凡纳滨对虾的健康管理和养殖提供依据。The composition,diversity,microbial predicted functions and interspecific interactions of the intestinal and hepatopancreatic flora between healthy shrimp and diseased shrimp infected with Hepatocystis hepatica were investigated comparatively using 16S rRNA high-throughput sequencing.The results showed that the diversity of bacterial communities in the intestinal and hepatopancreas was higher in healthy shrimp compared to affected shrimp.At the genus level,the composition and abundance of the dominant bacteria differed significantly in the intestine and hepatopancreas of healthy and diseased shrimp.In the intestine of healthy shrimp,Escherichia-Shigella and Spongiimonas were the dominant genera,while Shewanella,Megasphaera,Klebsiella,Pseudomonas and Halomonas were the dominant genera in the intestine of diseased shrimp.In the hepatopancreas of diseased shrimp Cupriavidus were the dominant genus.Tax4Fun2 predicted that the main functions of the intestinal and hepatopancreatic flora of healthy shrimp differed significantly from those of diseased shrimp.This study provides valuable insights into the effects of hepatic intestinal cysts on bacterial communities in the intestine and hepatopancreas of Litopenaeus vannameis,facilitating healthy management and culture of this shrimp species.

关 键 词：肠道菌群 16S rRNA测序 功能预测 凡纳滨对虾 

分 类 号：S941.5[农业科学—水产养殖]