南京市腹泻患者副溶血弧菌毒力基因及其耐药性分析  

Analysis of the virulence gene and drug resistance of Vibrio parahaemolyticus in diarrhea patients in Nanjing City

作　　者：张韶华[1] 江晓[1] 叶艳华[1] 史小超[1] 金萍[1] 雍玮[1] 王燕[1] 龚红瑾 王炜[1] 程婷婷[1] ZHANG Shaohua;JIANG Xiao;YE Yanhua;SHI Xiaochao;JIN Ping;YONG Wei;WANG Yan;GONG Hongjin;WANG Wei;CHENG Tingting(Nanjing Center for Disease Control and Prevention,Nanjing,Jiangsu 210003,China)

机构地区：[1]南京市疾病预防控制中心,江苏南京210003

出　　处：《医学动物防制》2023年第12期1123-1126,共4页Journal of Medical Pest Control

基　　金：江苏省卫生厅预防医学基金科研课题(Y2018003)。

摘　　要：目的分析南京市哨点医院急性腹泻患者副溶血弧菌携带的毒力基因及抗生素耐药性。方法收集2018—2021年哨点医院腹泻患者的肛拭子标本1805份,分离鉴定出74株副溶血弧菌,用PCR方法检测其毒力基因tlh、tdh、trh,并用微量肉汤法检测分离菌株的抗生素耐药性。用IBM SPSS 20软件进行统计分析,差异比较采用χ^(2)检验。结果74株副溶血弧菌tlh基因均为阳性,其中5.41%的菌株tdh(+)trh(+),86.49%的菌株tdh(+)trh(-),8.11%的菌株tdh(-)trh(-),三者差异有统计学意义(χ^(2)=141.243,P<0.001)。抗生素药敏试验结果显示100.00%的副溶血弧菌菌株对头孢唑啉耐药,21.62%的菌株对氨苄西林耐药,菌株普遍对三代头孢类抗生素(头孢噻肟、头孢他啶)及磺胺类、氨基糖苷类、四环素类等常用抗生素敏感,敏感率达90.00%以上。对硫霉素类抗生素(亚胺培南)的敏感率为100.00%。结论南京市腹泻患者粪便来源的副溶血弧菌毒力基因的携带率高,对常用抗生素较敏感,对一代头孢类抗生素(头孢唑啉)耐药严重。Objective To analyze the virulence gene and antibiotic resistance of Vibrio parahaemolyticus in patients with acute diarrhea in sentinel hospitals in Nanjing City.Methods A total of 74 strains of Vibrio parahaemolyticus were isolated and identified from 1805 anal swabs collected from diarrhea patients in sentinel hospitals from 2018 to 2021.The virulence genes tlh,tdh and trh were detected by PCR and the antibiotic resistance of the isolated strains was tested by micro broth method.Statistical analysis was performed using IBM SPSS 20 software,and differences were compared by theχ^(2) test.Results All 74 strains of Vibrio parahaemolyticus were positive for tlh gene,of which 5.41%of strains were tdh(+)trh(+),86.49%of strains were tdh(+)trh(-),8.11%of strains were tdh(-)trh(-).The difference between the three was statistically significant(χ^(2)=141.243,P<0.001).Antibiotic susceptibility test results showed that 100.00%of the Vibrio parahaemolyticus strains were resistant to cefazolin,21.62%of the strains were resistant to ampicillin,and the strains were generally sensitive to the third generation cephalosporin antibiotics(cefotaxime and ceftazidime),and sulfonamides,aminoglycosides,tetracyclines and other commonly used antibiotics,with a sensitivity rate of more than 90.00%.The sensitive rate to thiamphenicol antibiotics(imipenem)was 100.00%.Conclusion The virulence genes of Vibrio parahaemolyticus from fecal sources of diarrhea patients in Nanjing City has a high carrying rate,are more sensitive to commonly used antibiotics,and are severely resistant to the first generation cephalosporin antibiotics(cefazolin).

关 键 词：副溶血弧菌 腹泻 毒力基因 耐药性 分析 

分 类 号：R446.5[医药卫生—诊断学]