石墨烯及其衍生物对血管生成和血管化骨的影响机制  

Influential mechanism of graphene and its derivatives on angiogenesis and vascularized bone

作　　者：高丽 刘浏 任文燕 刘雪 王一宇 Gao Li;Liu Liu;Ren Wenyan;Liu Xue;Wang Yiyu(School of Stomatology,Jining Medical University,Jining 272067,Shandong Province,China;Jiangsu Provincial Key Laboratory of Oral Diseases Research,Nanjing Medical University,Department of Radiology,School of Stomatology,Nanjing Medical University,Nanjing 210029,Jiangsu Province,China)

机构地区：[1]济宁医学院口腔医学院,山东省济宁市272067 [2]南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学口腔医学院放射科,江苏省南京市210029

出　　处：《中国组织工程研究》2024年第17期2716-2722,共7页Chinese Journal of Tissue Engineering Research

基　　金：济宁市重点研发计划项目(2021YXNS032),项目负责人:刘雪;济宁医学院大学生创新训练计划项目(cx2021131),项目负责人:任文燕。

摘　　要：背景:石墨烯是最薄、最强韧的一类二维新型晶体材料,在生物医学的应用中显示出巨大的优势。血管生成和血管化骨是组织修复和再生的关键,是解决血管和成骨问题的有效途径。目的:综述石墨烯及其衍生物促进血管生成和血管化骨的特性及机制,为其在血管化组织修复与再生的临床应用提供参考。方法:利用计算机检索PubMed、ScienceDirect、中国知网和万方数据库收录的相关文献,中文检索词为“石墨烯,血管生成,血管化,血管化骨,内皮细胞”,英文检索词为“graphene,Angiogenesis OR Vascularization,Vascularized bone,endothelial cells”。排除与文章主题不相关的文献,根据纳入标准和排除标准,最终纳入62篇文献进行结果分析。结果与结论:①目前氧化石墨烯在石墨烯及其衍生物中的研究较多,应用最为广泛。②石墨烯及其衍生物适用于心脏、骨、神经和创伤愈合等相关疾病。③石墨烯及其衍生物有优异的理化性能和生物学性能,但其存在潜在的细胞毒性,在应用中需注意其生物安全性。④石墨烯及其衍生物的应用还需要进一步的研究来证明其最适尺寸和浓度及降低毒性的措施。⑤就细胞层面而言,石墨烯及其衍生物可以通过促进尖端内皮细胞表型、间充质干细胞黏附和增殖、血管平滑肌细胞的生长,从而促进血管生成活性。⑥就分子层面而言,石墨烯及其衍生物可以增加血管内皮生长因子、碱性成纤维细胞生长因子及肝细胞生长因子等的表达并活化活性氧/一氧化氮合酶/一氧化氮信号通路、溶血磷脂酸R6/Hippo-YAP通路、基质细胞衍生因子1/血管内皮生长因子和ZEB1/Notch1通路。⑦氧化石墨烯和氧化石墨烯-铜可以磷酸化细胞外调节蛋白激酶并激活缺氧诱导因子1,进而促进血管内皮生长因子和骨形态生成蛋白2的表达上调,从而促进血管生成和血管化骨。⑧因此,石墨烯及其�BACKGROUND:Graphene is the thinnest,strongest,and toughest type of two-dimensional new crystal material,demonstrating significant advantages in biomedical applications.Angiogenesis and vascularization of bone are key factors in tissue repair and regeneration,and are effective ways to address vascular and osteogenic issues.OBJECTIVE:To review the characteristics and mechanisms of graphene and its derivatives in promoting angiogenesis activity and vascularizing bone,in order to provide a reference for their clinical application in vascular tissue repair and regeneration.METHODS:Using a computer to search for relevant literature included in PubMed,ScienceDirect,CNKI,and Wanfang databases,the Chinese search terms were“grapheme”,“angiogenesis,vascularization”,“vascularized bone”,and“endothelial cells”,while the English search terms were“graphene”“angiogenesis OR vascularization”“vascularized bone”“endothelial cells”.After excluding literature unrelated to the topic of the article,according to the inclusion and exclusion criteria,62 articles were ultimately included for result analysis.RESULTS AND CONCLUSION:(1)At present,graphene oxide has been studied more and is the most widely used in graphene and its derivatives.(2)Graphene and its derivatives are suitable for heart,bone,nerve,and wound healing related diseases.(3)Graphene and its derivatives have excellent physical and chemical properties and biological properties,but they have potential cytotoxicity.We should pay attention to its biological safety in application.(4)The application of graphene and its derivatives requires further research to demonstrate the optimal size and concentration and measures to reduce toxicity.(5)On the cellular level,graphene and its derivatives can promote angiogenic activity by tip endothelial cell phenotype,mesenchymal stem cell adhesion and proliferation,and vascular smooth muscle cell growth.(6)On the molecular level,graphene and its derivatives can increase the expression of vascular endothelial gro

关 键 词：石墨烯及其衍生物 血管化 血管生成 血管化骨 内皮细胞 支架材料 分子机制 信号通路 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R363