0.53%瑞香素乳油诱导烟草抗青枯病的效果  

The Effects of 0.53% Daphnetin EC on Inducing Tobacco Resistance to Tobacco Bacterial Wilt

作　　者：李吉秀 朱晓伟[2] 王叶 王垚 杨亮[1] 徐小洪[2] LI Jixiu;ZHU Xiaowei;WANG Ye;WANG Yao;YANG Liang;XU Xiaohong(School of Plant Protection,Southwest University,Chongqing 400715,China;Chongqing Company of China Tobacco Corporation,Chongqing 400023,China)

机构地区：[1]西南大学植物保护学院,重庆400715 [2]中国烟草总公司重庆市公司,重庆400023

出　　处：《植物医学》2023年第5期33-40,共8页Plant Health and Medicine

基　　金：重庆市烟草专卖局科技项目(B20211NY1316);渝东南植烟土壤改良关键技术集成与示范应用(B20221NY1312)。

摘　　要：为明确0.53%瑞香素乳油对烟草青枯病的防效和防病机制,通过室内盆栽试验、酶活性测定和田间试验,分析0.53%瑞香素乳油对烟草青枯防控效果及叶片防御酶酶活性.室内结果表明,与0.1%S-诱抗素水剂、5%氨基寡糖素水剂、0.01%芸苔素内酯乳油相比,0.53%瑞香素乳油能够有效减少烟草青枯病的发生,在接种后第10 d,诱导抗性效果依然高达71.66%.瑞香素可显著提高烟叶PPO酶、CAT酶和SOD酶活性,其酶活性分别在10 min、12 h和24 h达到最大值,其值分别为169.02 U/g,145.42 U/g和111.51 U/g.田间试验结果显示,0.53%瑞香素乳油能够促进烟株生长,而且在病害发生高峰期(8月7日),0.53%瑞香素乳油能够有效诱导烟草对青枯病的抗性,相对防效达到44.33%,优于商品类抗性诱导剂.以上结果表明,0.53%瑞香素乳油可作为一种新型的植物源抗性诱导剂,为烟草青枯病的绿色防控提供理论支撑.In order to explore the role of daphnetin as a potential new plant elicitor,and clarify control effect of daphnetin on tobacco bacterial wilt,several experiments such as pot experiment,enzyme activity determination and field experiment were performed in this assay.The results showed that daphnetin could effectively reduce the occurrence of tobacco bacterial wilt compared with other plant defense elicitors.The efficiency of induced resistance by daphnetin was still up to 71.66%after 10 days of inoculation with Ralstonia solanacearum.Daphnetin can significantly increase the activities of superoxide dismutase,catalase and polyphenol oxidase in tobacco leaves,and the enzyme activities reached to the maximum value at 10 min,12 h and 24 h,at 169.02 U/g,145.42 U/g and 111.51 U/g,respectively.Field experiment showed that daphnetin promoted tobacco growth,and during outbreak period of bacterial wilt disease,daphnetin significant induced systemic resistance of tobacco with a control efficiency of 44.33%.These results showed that daphnetin can be considered as a plant-derived resistance inducer for controlling tobacco bacterial wilt,which could provide theoretical support for the green prevention and control of plant disease.

关 键 词：瑞香素 烟草青枯病 防效 酶活性 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]