The study on effect of long-termed administration of mixed rare earth Changle on rat liver  

作　　者：LIU Ying,CHEN Dong ,CHEN Ai jun,WANG Xiao ming,NIE Yu xiu (Dept. of Histology and Embryology,School of Basic Medical Sciences,Jilin University,Changchun 130021, China) 

出　　处：《吉林大学学报（医学版）》2002年第6期567-570,共4页Journal of Jilin University：Medicine Edition

基　　金：Project supported by the National Natural Science Foundation of China (2 9890 2 80 - 3)

摘　　要：目的 :研究不同剂量的“常乐”对大鼠肝脏结构和功能的影响。方法 :采用健康 Wistar大鼠 1 80只 ,每组 30只 ,实验组分别以 0 .1、0 .2、2 .0、1 0 .0、2 0 .0 mg· kg-1常乐连续灌胃 6个月 ,每日 1次。应用常规组织化学、透射电镜技术及血清生化测定技术 ,观察动物肝脏的结构和功能变化。结果 :各实验组动物体重随染毒剂量的减少而呈线性增长。 2 0 .0 mg·kg-1组肝细胞内糖原颗粒减少 ,肝细胞核有不同程度的变形 ,门管区有炎细胞浸润 ,其余各组肝脏结构与对照组相比无明显改变。各实验组动物肝脏 Kupffer细胞及肝细胞中均可见到高电子密度的致密体及含电子密度较高颗粒的溶酶体 ,其数量随染毒剂量的增大而增多。 2 0 .0 mg· kg-1组血清 ALP及 GPT增高。结论 :2 0 .0 mg·kg-1“常乐”长期作用对大鼠肝脏结构和功能均有损害。Objective: To research effect of different doses of Changle on the structure and function of rat liver.Methods: 180 healthy Wistar rats were divided into 5 experimental groups according following doses: 0.1,0.2,2.0,10.0 and 20.0 mg·kg -1 ,respectively, and the control group given physiological saline for six months. The changes of liver structure were examined by means of normal histological chemistry and transmission electron microscope(TEM). Results: The body weight of animal was linearly increased with the decrease of administered doses, gradual reduction of glycogen in hepatocytes and infiltration of inflammatory cells in the portal area were found in the group of 20.0 mg·kg -1 . Changes of ultrastructure showed there were dense bodies and lysosomes containing dense granules in Kupffer cell and hepatocyte,and they were increased along with doses adding. Nuclei deformed, ALP and GPT in serum were rose in the group of 20.0 mg·kg -1 . Different doses of Changle could lead to distinct biological effects. Conclusion: Long termed administration of 20.0 mg·kg -1 Changle can lead to damage of structure and function of rat liver.

关 键 词：混和稀土制剂 常乐 长期用药 鼠肝 影响 肝功能 

分 类 号：R977.9[医药卫生—药品]