石油烃降解菌还原六价铬的特性及条件优化研究  

Characteristics and conditions optimization of hexavalent chromium reduction by a petroleum hydrocarbon degradation strain

作　　者：王广顺 张皓璠 唐晨[1] 郭东北[1] 刘垂伟 赵苒[1] WANG Guang-shun;ZHANG Hao-fan;TANG Chen;GUO Dong-bei;LIU Chui-wei;ZHAO Ran(State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Public Health,Xiamen University,Xiamen 361102,China;State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210023,China)

机构地区：[1]厦门大学公共卫生学院分子疫苗学和分子诊断学国家重点实验室,福建厦门361102 [2]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210023

出　　处：《现代化工》2023年第11期99-103,110,共6页Modern Chemical Industry

基　　金：国家自然科学基金面上项目(81673129);厦门市自然科学基金面上项目(3502Z20227178);厦门大学大学生创新创业训练计划项目(202210384047)。

摘　　要：通过对石油烃降解菌Paenarthrobacter ureafaciens Thp2-23进行16S rRNA基因测序分析,利用MEGA 7.0构建菌株系统发育树,并采用二苯碳酰二肼分光光度法检测Thp2-23在不同条件下对Cr(Ⅵ)的还原率;采用SPSS 25.0对各组还原率进行统计学描述及方差分析,探讨pH、温度、石油烃、吐温80和铜离子对菌株还原Cr(Ⅵ)效果的影响。结果表明,菌株Thp2-23在pH 8.0、40℃的最优还原条件下,12 h对50 mg/L Cr(Ⅵ)的还原率为95%,24 h对Cr(Ⅵ)的还原率达到100%。石油烃和吐温80可抑制Thp2-23对Cr(Ⅵ)的还原,铜离子可促进Thp2-23对Cr(Ⅵ)的还原。A 16S rRNA gene sequencing analysis is done on Paenarthrobacter ureafaciens Thp2-23,a petroleum hydrocarbon degradation strain.The strain phylogenetic tree is constructed by using MEGA 7.0.Diphenylcarbazide spectrophotometric method is employed to detect the reduction rate of Cr(Ⅵ)by Thp2-23 under different conditions.By means of SPSS 25.0 software,descriptive statistical analysis and variance analysis are performed on the reduction rates of each group.The influence of pH,temperature,petroleum hydrocarbons,Tween-80 and copper ion on the effectiveness of Cr(Ⅵ)reduction by the strain is explored.The results indicate that under the optimal reduction conditions including a pH of 8.0 and 40℃,the reduction rate of 50 mg·L^(-1)Cr(Ⅵ)by Thp2-23 strain reaches 95%within 12 h,and 100%within 24 h.Cr(Ⅵ)reduction may be inhibited by petroleum hydrocarbon and Tween 80,but promoted by copper ion.

关 键 词：微生物修复 六价铬 石油烃 

分 类 号：X53[环境科学与工程—环境工程]