葡萄蔗糖转化酶基因家族的生物信息学及响应逆境胁迫分析  

Bioinformatics Analysis of Grape Invertase Gene Family and Response to Adversity Stress

作　　者：刘铭 杨尚雯 马维峰 卢世雄 梁国平 毛娟[1] LIU Ming;YANG Shangwen;MA Weifeng;LU Shixiong;LIANG Guoping;MAO Juan(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,兰州730070

出　　处：《西北植物学报》2023年第11期1801-1812,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点研发计划项目(2022YFD1602106);甘肃省农业科技支撑项目(KJZC-2013-13)。

摘　　要：蔗糖转化酶(invertase,INV)在植物生长发育和抵御胁迫中发挥着重要作用。研究从葡萄基因组数据库中鉴定出19个蔗糖转化酶基因,对基因结构和编码蛋白质的理化性质进行生物信息学分析,并利用qRT-PCR技术分析基因在不同激素和非生物胁迫条件下的表达特征,为进一步探索葡萄INV基因家族参与葡萄逆境响应提供了一定的理论依据。结果表明,(1)该基因家族编码蛋白的氨基酸长度在150~766 aa之间,理论等电点介于4.43~9.1之间,亚细胞定位预测发现其主要在细胞质中表达,此外液泡和细胞壁也存在部分基因表达;(2)共线性结果显示VvCINV与其他5个物种复制频率较高;(3)保守基序分析表明VvCwINV包含了所有的保守基序,且Glyco_32和Glyco_hydro_100是VvINV基因主要结构域;(4)组织特异性表达分析发现多数基因在葡萄生长发育进程中都有表达;(5)qRT-PCR分析结果显示,VvINV基因家族在叶片中对激素处理和非生物胁迫的响应出现上调,VvCINV1在50 mg/L GA_(3)和10%PEG处理后上调表达极显著,VvCINV4在盐胁迫、ABA和低温处理后也上调表达显著。这些研究结果表明,VvINVs可能调控葡萄生长发育过程中激素和对非生物胁迫的响应。Invertase(INV)plays an important role in the plant growth and development and resistance to the stress.In this study,19 INV family members were identified in the grape genome database,and the structures of the genes and the physical-chemical properties of the coding proteins were analyzed by bioinformatics.The expression characteristics of the genes under different hormone and abiotic stress conditions were analyzed by fluorescence quantitative PCR(qRT-PCR)technology,which could provide a theoretical basis for further exploring the involvement of VvINV gene family in grape stress response.The results were as followings,(1)the gene family encoded proteins with amino acid lengths between 150766 aa and the theoretical isoelectric point ranged from 4.43 to 9.1.(2)Collinearity analysis showed that VvCINVs had a higher replication frequency with other 5 species.(3)The conserved motifs analysis showed that VvCwINVs contained all conserved motifs,and Glyco_32 and Glyco_hydro_100 were the main domains of VvINV genes.(4)Tissue-specific expression analysis showed that the most genes were expressed during the growth and development of grape.(5)The results of qRT-PCR showed that VvINV family genes were up-regulated in response to hormone treatments and abiotic stresses in leaves;VvCINV1 was highly expressed under 50 mg/L GA_(3)and 10%PEG treatments;VvCINV4 was significantly expressed under salt,ABA and low temperature treatments.These results indicated that VvINVs could regulate hormone and abiotic stress response during grape growth and development.

关 键 词：葡萄 INV基因家族 生物信息学 QRT-PCR 激素 非生物胁迫 

分 类 号：Q949.9[生物学—植物学] S663.1[农业科学—果树学]