In Situ Deposition of Drug and Gene Nanoparticles on a Patterned Supramolecular Hydrogel to Construct a Directionally Osteochondral Plug  

作　　者：Jiawei Kang Yaping Li Yating Qin Zhongming Huang Yifan Wu Long Sun Cong Wang Wei Wang Gang Feng Yiying Qi 

机构地区：[1]Department of Orthopaedic Surgery,The Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou City 310009,Zhejiang Province,People’s Republic of China [2]College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,People’s Republic of China [3]ZJU-Hangzhou Global Scientific and Technological Innovation Center,Hangzhou 311215,Zhejiang,People’s Republic of China [4]The Affiliated Nanhua Hospital,Orthopedic Research Centre,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,People’s Republic of China [5]Department of Radiology,Jining No.1 People’s Hospital,Jining Medical University,Jining 272000,Shandong,People’s Republic of China

出　　处：《Nano-Micro Letters》2024年第1期341-359,共19页纳微快报（英文版）

基　　金：the Natural Science Foundation of China(Grant Nos.82072413,82101649);National Key Research and Development Program of China(Grant Nos.2021YFE0105400).

摘　　要：The integrated repair of bone and cartilage boasts advantages for osteochondral restoration such as a long-term repair effect and less deterioration compared to repairing cartilage alone.Constructing multifactorial,spatially oriented scaffolds to stimulate osteochondral regeneration,has immense significance.Herein,targeted drugs,namely kartogenin@polydopamine(KGN@PDA)nanoparticles for cartilage repair and miRNA@calcium phosphate(miRNA@CaP)NPs for bone regeneration,were in situ deposited on a patterned supramolecular-assembled 2-ureido-4[lH]-pyrimidinone(UPy)modified gelation hydrogel film,facilitated by the dynamic and responsive coordination and complexation of metal ions and their ligands.This hydrogel film can be rolled into a cylindrical plug,mimicking the Haversian canal structure of natural bone.The resultant hydrogel demonstrates stable mechanical properties,a self-healing ability,a high capability for reactive oxygen species capture,and controlled release of KGN and miR-26a.In vitro,KGN@PDA and miRNA@CaP promote chondrogenic and osteogenic differentiation of mesenchymal stem cells via the JNK/RUNX1 and GSK-3β/β-catenin pathways,respectively.In vivo,the osteochondral plug exhibits optimal subchondral bone and cartilage regeneration,evidenced by a significant increase in glycosaminoglycan and collagen accumulation in specific zones,along with the successful integration of neocartilage with subchondral bone.This biomaterial delivery approach represents a significant toward improved osteochondral repair.

关 键 词：Osteochondral regeneration Oriented hydrogel Kartogenin miRNA-26a 

分 类 号：O62[理学—有机化学]