参附注射液对广西巴马小型猪离体空跳心脏心肌保护的研究  

Study of Shenfu injection on myocardial protection of isolated beating heart of Guangxi Bama miniature pigs

作　　者：刘豫 高春雁 武伟[2] 覃冠斌 罗兰[2] 冯志强[3] 银世杰[2] LIU Yu;GAO Chunyan;WU Wei(Guangxi University of Chinese Medicine,Guangxi,Nanning 530200,China;不详)

机构地区：[1]广西中医药大学,南宁市530200 [2]广西中医药大学第一附属医院东葛院区麻醉科 [3]广西中医药大学第一附属医院东葛院区胸心血管外科

出　　处：《河北医药》2023年第23期3554-3558,共5页Hebei Medical Journal

基　　金：国家自然科学基金资助项目(编号:81960799);广西中医药大学B类“高层次人才培育创新团队”项目(编号:2022B007)。

摘　　要：目的探讨参附注射液对广西巴马小型猪离体空跳心脏模型的心肌保护作用。方法将12头体重25~30 kg的健康成年广西巴马小猪随机分成参附组(n=6)和0.9%氯化钠溶液组(n=6),将灌注了心脏停跳液(HTK液)的心脏与体外循环机连接,常温离体灌注8 h,比较灌注第0个小时(T0)、第1个小时(T1)、第2个小时(T2)、第3个小时(T3)、第4个小时(T4)、第5个小时(T5)、第6个小时(T6)、第7个小时(T7)、第8个小时(T8)9个时间点灌注液中肌酸激酶同工酶(CK-MB),乳酸脱氢酶(LDH),α-羟丁酸脱氢酶(α-HBDH)的水平,同时监测9个时间点的心率、灌注压和每分钟流量以及T0、T4、T83个时间点灌注液中IL-6、TNF-α、IL-8的水平;通过透射电镜观察灌注第8个小时心脏停搏后左心室壁心肌组织的超微结构。结果2组灌注液中CK-MB、α-HBDH、LDH的水平随着时间变化显著增加(P<0.05);和0.9%氯化钠溶液组比较,参附注射液组灌注液中9个时间点的CK-MB,α-HBDH,LDH水平明显降低,差异有统计学意义(P<0.05)。TNF-α、IL-6、IL-8在灌注液中的浓度随时间显著增加(P<0.05),参附注射液组灌注液中TNF-α、IL-6、IL-8的浓度明显低于0.9%氯化钠溶液组,差异有统计学意义(P<0.05),但2组心率、灌注压和每分钟流量无明显差异(P>0.05)。和0.9%氯化钠溶液组比较,参附组心肌组织损伤程度较轻。结论参附注射液对广西巴马小型猪离体空跳心脏的心肌细胞具有保护作用。Objective To explore the myocardial protective effect of Shenfu injection on the isolated beating heart model of Guangxi Bama miniature pigs(Sus scrofa).Methods Twelve healthy adult Guangxi Bama miniature pigs weighing 25~30kg were randomly divided into the Shenfu group(n=6)and the normal saline group(n=6).The hearts perfused with cardioplegia solution(HTK solution)were connected to the extracorporeal circulation machine,and perfused in vitro at room temperature for 8h.Creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH)andα-hydroxybutyrate dehydrogenase(α-HBDH)in the perfusion solution at 9 time points:0(T0),1h(T1),2h(T2),3h(T3),4h(T4),5h(T5),6h(T6),7h(T7),and 8h(T8)of perfusion were measured.The heart rate,perfusion pressure and flow rate per minute at 9 time points,as well as interleukin 6(IL-6),IL-8 and tumor necrosis factor-alpha(TNF-α)levels in the perfusion solution at T0,T4 and T8 were measured.The ultrastructure of left ventricular wall myocardial tissue after cardiac arrest at T8 was observed by transmission electron microscopy.Results CK-MB,LDH andα-HBDH levels in the perfusion solution significantly increased with time in both groups(P<0.05).Their levels at 9 time points were significantly lower in Shenfu group than those of normal saline group(P<0.05).IL-6,IL-8 and TNF-αlevels in the perfusion solution significantly increased with time in both groups(P<0.05),and their levels were significantly lower in Shenfu group than those of normal saline group(P<0.05).There were no significant differences in heart rate,perfusion pressure and flow per minute between the two groups(P>0.05).Compared with the normal saline group,myocardial tissue damage in Shenfu group was milder.Conclusion Shenfu injection has a protective effect on myocardial cells of isolated beating heart of Guangxi Bama miniature pigs.

关 键 词：参附注射液 体外循环 心肌保护 离体空跳心脏 供心保存 

分 类 号：R617[医药卫生—外科学]