羔羊不同超排方法的筛选及其对卵母细胞体外成熟的影响  

Screening of superovulation methods in lambs and their effects on oocyte maturation in vitro

作　　者：刘昱成 王晶晶[1,2] 张振良 倪建宏 刘长彬[1,2] 傅祥伟 石国庆[1,2] 万鹏程 崔炳灿 LIU Yucheng;WANG Jingjing;ZHANG Zheniang;NI Jianhong;LIU Changbin;FU Xiangwei;SHI Guoqing;WAN Pengcheng;CUI Bincan(Animal Husbandry and Veterinary Institute,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832000,China;State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Shihezi 832000,China;Xinjiang Agricultural Vocational and Technical College,Changji 831100,China)

机构地区：[1]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [2]省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000 [3]新疆农业职业技术学院,新疆昌吉831100

出　　处：《畜牧与兽医》2023年第12期8-12,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家绒毛用羊产业技术体系(CARS-39-07);国家重点研发计划(2020YFD1300900);兵团科技特派员创新创业计划项目(2022CB026);新疆农垦科学院院级科研项目(2023YJ010);八师创新环境建设和能力提升计划项目(2023RC07)。

摘　　要：旨在比较不同超排方案对羔羊超数效果及其对卵母细胞体外成熟的效果,筛选最佳幼畜超排方案。试验以1~2月龄绵羔羊为研究对象,分为3组,其中2组(方案I和方案II注射激素)为试验超排组,1组为对照组(未注射激素),探究不同超排方法对绵羔羊卵巢的反应性、卵巢的大小及卵母细胞体外成熟的影响。结果表明,6次×12 h间隔、注射总剂量180 U促卵泡素(FSH)的超排方案Ⅱ效果优于4次×12 h间隔、注射总剂量200 U FSH的超排方案Ⅰ,超排方案Ⅱ获得发育卵泡数(348.00±8.25)枚/只优于超排方案Ⅰ发育卵泡数(265.00±6.32)枚/只;体外培养卵母细胞24 h后,超排方案Ⅱ卵母细胞成熟率45.4%优于超排方案Ⅰ卵母细胞成熟率32.2%。研究筛选出6次×12 h间隔、注射总剂量180 U FSH,第1次注射FSH时同时肌内注射330 U PMSG的超排方案为最佳幼畜超排方案。本试验结果为绵羔羊扩展体外受精所需卵子的来源,缩短世代间隔,产生大量优秀后代,加速育种进程等方向的研究奠定一定的理论基础。This study was to compare the effects of different superovulation protocols on lamb supernumeraries and on their oocyte maturation in vitro and to screen the best superovulation protocol for the young animals.The experiment was conducted on 1-2 month old sheep lambs which were divided into three groups,two of which,the scheme Ⅰ and scheme Ⅱ groups,were injected with hormones as the experimental superovulation groups and one of which was the control group without hormone injection,so as to determine the effects of different superovulation methods on ovarian responsiveness,ovarian size and oocyte maturation in vitro in sheep lambs.The results showed that the ovulation protocol Ⅱ groups treated with 6×12 h intervals and a total dose of 180 U FSH was more effective than the ovulation protocol Ⅰ group with 4×12 h intervals and a total dose of 200 U FSH.The oocyte maturation rate was 45.4% in the superovulation Ⅱ group,as compared to 32.2% in the superovulation Ⅰ group.To conclude,screening of a superovulation protocol with 6×12 h intervals,a total dose of 180 U FSH and 330 U PMSG injected intramuscularly at the same time as the first FSH injection was the best superovulation protocol for the young animals,which laid a theoretical foundation for sheep lambs in expanding the source of eggs needed forin vitrofertilization,in shortening the generation interval,in producing a large number of excellent offspring and in speeding up the breeding process.

关 键 词：绵羔羊 体外成熟 卵母细胞 超数排卵 

分 类 号：S814[农业科学—畜牧学]