应用Cephodex 微载体培养DF-1细胞增殖水貂源犬瘟热病毒的技术研究  

Investigation into the technology of culturing DF-1 to proliferate canine distemper virus isolated from mink with Cephodex micro-carrier

作　　者：梅建国[1,2] 庄金秋 任强[3] 莫玲 王艳[1] 李峰[1] 郭时金[1] 付石军[1] 赵蕾[1] 王建军 MEI Jianguo;ZHUANG Jinqiu;REN Qiang;MO ling;WANG Yan;LI Feng;GUO Shijin;FU Shijun;ZHAO Lei;WANG Jianjun(Binzhou Academy of Animal Science&Veterinary Medicine,Binzhou 256600,China;Binzhou Bio-carrier Biotechnology Co,Ltd,Binzhou 256600,China;Hospital Affiliated to Binzhou Medical University,Binzhou 256600,China)

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]滨州贝尔凯瑞生物技术有限公司,山东滨州256600 [3]滨州医学院附属医院,山东滨州256600

出　　处：《畜牧与兽医》2023年第12期62-67,共6页Animal Husbandry & Veterinary Medicine

基　　金：山东省生猪产业技术体系(SDAIT-08-13)。

摘　　要：为了建立水貂源犬瘟热病毒(CDV)的大规模悬浮培养技术,实现细胞高密度生长和病毒高效增殖,本研究应用Cephodex微载体悬浮培养鸡胚成纤维细胞系DF-1细胞,增殖弱毒株CDV3。整个过程采用摇瓶培养法,通过对病毒培养温度、病毒收获时间等关键技术条件进行优化,确立最佳培养条件。结果表明,DF-1细胞37℃培养至72 h,接种CDV3,35℃继续培养72 h收获病毒,病毒滴度每0.1 mL可达10^(5.0) TCID_(50)。CDV微载体悬浮培养技术的初步建立,为高效水貂犬瘟热疫苗的研发生产奠定了重要的基础。In order to establish a large-scale suspension culture technology of canine distemper virus(CDV) isolated from mink and realize its high-density cell growth and efficient virus proliferation,Cephodex was used to culture DF-1 cells by suspension to proliferate CDV3.In the whole process,shake flask culture was adopted,and optimal culture conditions were established by optimizing the key technical conditions such as virus culture temperature and virus harvest time.The results showed that,after being cultured at 37 ℃ for 72 hours,and the DF-1 cells were inoculated with CDV3.Then,these cells were infected by the CDV3 and continued to be cultured at 35 ℃ for 72 hours,and finally the viral titer reached 10^(5.0) TCID_(50) per 0.1 mL.Therefore,a technology of CDV micro-carrier suspension culture was established here,which laid an important technical foundation for high-efficiency mink canine distemper vaccine production.

关 键 词：犬瘟热病毒 微载体 悬浮培养 DF-1细胞 疫苗 

分 类 号：S852[农业科学—基础兽医学]