无距虾脊兰根际土壤真菌与根系内生真菌多样性  

Diversity of rhizosphere soil fungi and root endophytic fungi of Calanthe tsoongiana

作　　者：何水莲 黄蓓 李田园 田敏[1] HE Shuilian;HUANG Bei;LI Tianyuan;TIAN Min(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,Zhejiang,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [2]南京林业大学林学院,江苏南京210037

出　　处：《浙江农林大学学报》2023年第6期1158-1166,共9页Journal of Zhejiang A&F University

基　　金：浙江省农业(花卉)新品种选育重大科技专项(2021C02071-5);浙江省重点研发计划项目(2019C02036)。

摘　　要：【目的】研究野生无距虾脊兰Calanthe tsoongiana在萌芽期、花期、果期、衰亡期根际土壤真菌和根系内生真菌多样性差异,旨在明确不同生长发育时期真菌多样性特征,为无距虾脊兰种群扩繁提供理论依据。【方法】采用内转录间隔区(internal transcribed spacer,ITS)测序技术,分析野生无距虾脊兰4个时期根际土壤真菌与根系内生真菌群落的物种组成及相对丰度变化,找出各时期优势真菌。【结果】4个时期共获得307288条优化序列,平均序列长度为643 bp,隶属16门68纲176目413属。萌芽期根际土壤真菌和根系内生优势真菌为分别被孢霉属Mortierella和红菇属Russula,花期优势真菌分别为青霉属Penicillium和粗糙孔菌属Trechispora,果期分别为Paraboeremia和蜡壳耳属Sebacina,衰亡期分别为Paraboeremia和镰孢属Fusarium。Alpha多样性指数显示:根系内生真菌多样性和相对丰度从萌芽期到果期依次递增,在果期达到顶峰,衰亡期又急剧下降。根际土壤真菌中花期多样性最高,其余3个时期变化相对平稳。【结论】无距虾脊兰根际土壤真菌和根系内生真菌多样性和丰富度在整个生长发育时期存在较大差异,花期和果期真菌多样性高于衰亡期和萌芽期。[Objective]This study,with an investigation into the diversity differences of rhizosphere soil fungi and root endophytic fungi in the wild population of Calanthe tsoongiana,is aimed to clarify the diversity characteristics of fungi in different growth and development stages so as to provide theoretical basis for population propagation.[Method]Internal transcribed spacer(ITS)sequencing technology was used to analyze the species composition and abundance changes of endophytic fungi communities in the rhizosphere soil and roots at the four stages of C.tsoongiana with the dominant fungi at each stage investigated.[Result]A total of 307288 optimized sequences were obtained from the four periods,with an average length of 643 bp,belonging to 16 phyla,68 classes,176 orders,and 413 genera.At the germination stage,the dominant fungi in rhizosphere soil and root were Mortierella and Russula,respectively;Penicillium and Trechispora were the dominant fungi at the flowering stage;at the fruiting stage,the dominant fungi were Paraboeremia and Sebacina while Paraboeremia and Fusarium were the dominant fungi at the decay stage.The results of Alpha diversity index showed that the diversity and relative abundance of endophytic fungi increased from the germination stage to the fruiting stage,reaching the peak at the fruiting stage,and decreasing sharply at the declining stage.The diversity of rhizosphere soil fungi was the highest at the flowering stage,and the changes in the other three stages were relatively stable.[Conclusion]The diversity and richness of rhizosphere soil fungi and root endophytic fungi were significantly different during the whole growth and development period whereas flowering and fruiting fungal diversity was higher than in budding and dying.This study has provided an important reference for artificial breeding and conservation of wild resources in C.tsoongiana.[Ch,5 fig.2 tab.34 ref.]

关 键 词：无距虾脊兰 内生真菌 多样性 不同时期 

分 类 号：S718.81[农业科学—林学]