海水养殖中华鲟后备亲本的血液生化特征  

Blood biochemical characteristics of reserve parents of maricultured Acipenser sinensis

作　　者：李远兰 张涛[2,3,4] 冯广朋 庄平 章龙珍[2,3,4] 杨刚[2,3,4] 唐琼英 LI Yuanan;ZHANG Tao;FENG Guangpeng;ZHUANG Ping;ZHANG Longzhen;YANG Gang;TANG Qiongying(School of Life Science,Zhejiang Huzhou University,Huzhou Zhejiang 313000,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Shanghai Engineering Research Center of Fisheries Resources Enhancement and Ecological Restoration of the Yangtze Estuary,Shanghai 200090,China;Scientific Observing and Experimental Station of Fisheries Resources and Environment of East China Sea and Yangtze Estuary,Ministry of Agriculture and Rural Affairs,Shanghai 200090,China)

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000 [2]中国水产科学研究院东海水产研究所,上海200090 [3]上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090 [4]农业农村部东海与长江口渔业资源环境科学观测实验站,上海200090

出　　处：《海洋渔业》2023年第5期570-578,共9页Marine Fisheries

基　　金：国家重点研发计划(2020YFD0900805);修复向家坝库区渔业资源及保护长江珍稀特有物种第二期项目(QT-20036);2022年物种资源保护项目-中华鲟江海统筹保护(A060104)。

摘　　要：为探究海水养殖条件下中华鲟(Acipenser sinensis)后备亲本的生理状况,对海水养殖5年的13尾中华鲟后备亲本的28项血液生化指标进行了测定。结果表明:中华鲟血液谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)和总胆红素(total bilirubin,TBIL)含量分别为(1.48±0.93)U·L^(-1)、(45.70±19.07)U·L^(-1)和(0.83±0.39)μmol·L^(-1),尿酸(uric acid,UA)和肌苷(creatinine,CR)浓度分别为(11.58±8.80)μmol·L^(-1)和(15.98±5.12)μmol·L^(-1);28项血液生化指标中,葡萄糖(glucose,GLU)、甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白(low density lipoprotein cholesterol,LDL-C)、白蛋白(albumin,ALB)、球蛋白(globulin,GLB)、白/球蛋白比(A/G)、总蛋白(total protein,TP)、CR、TBIL、AST、γ-谷胺酰基转移酶(gamma-glutamyltransferase,GGT)、胆碱酯酶(cholinesterase,CHE)、碱性磷酸酶(alkaline phosphatase,ALP)、乳酸脱氢酶(lactate dehydrogenase,LDH)等15项指标变异系数小于0.5;高密度脂蛋白(high density liptein cholesterol,HDL-C)、载脂蛋白B(apolipoprotein B,APO-B)、总胆汁酸(total bile acid,TBA)、尿素氮(blood urea nitrogen BUN)、UA、直接胆红素(direct bilirubin,DBIL)、间接胆红素(indirect bilirubin,IBIL)、ALT、谷草/谷丙转氨酶比(AST/ALT)、α-羟丁酸脱氢酶(α-hydroxybutyrate dehydrogenase,α-HBDH)、肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(creatine kinase-MB,CK-MB)等12项指标变异系数在0.5~1.0之间;仅载脂蛋白A1(apolipoprotein A1,APO-A1)的变异系数大于1.0。与淡水养殖的中华鲟生化指标相比,海水养殖条件下的中华鲟血液的ALT、AST和TBIL活性有所降低,揭示其肝脏代谢可能产生了适应性变化;各项血液生化指标变异系数均较大,表明相同年龄不同个体之间血液生化指标的差异较大。研究结果可为海水养殖中华鲟的血液生化指标提供参考,并丰富海水养殖中华鲟血液生化的�Chinese sturgeon(Acipenser sinensis)has extremely important ecological and scientific value.Due to human interference,its wild population is on the brink of extinction.The life history of Acipenser sinensis is relatively complex,before the closure of the Gezhouba Dam on the Yangtze River,sexually mature individuals entered the Yangtze River Estuary every July to August to begin their anadromous reproductive migration.In October to November of the next year,the spawning colonies migrate to the spawning grounds in Jinsha River section of the upper reaches of the Yangtze River to breed.The spawned larvae drift down with the river water,arrive in the Yangtze River Estuary in June of the next year,stay in the Yangtze River Estuary for about four months to fatten,and then migrate to the sea.After more than 10 years of growth and development in the sea,they reach gonadal maturity,enter the upper reaches of the Yangtze River to breed and repeat the cycle.The marine life history of Acipenser sinensis accounts for approximately 80%of the total life history,therefore,strengthening the protection of the marine life history stage is of great significance for the protection of Acipenser sinensis species.At present,artificial storage is one of the important methods for preserving the species of Acipenser sinensis,and freshwater aquaculture technology has formed a relatively mature technical system.However,current research on the Acipenser sinensis living in seawater is very limited,with only some reports on its feeding habits and population distribution.Research on the aquaculture of Acipenser sinensis in seawater is almost blank.This experiment aims to further understand the physiological status of Acipenser sinensis under seawater storage conditions by studying the blood biochemical indicators of its reserve parents under seawater aquaculture conditions,providing basic data for biological research and“land sea land relay aquaculture”of Acipenser sinensis.In order to explore the physiological status of reserve parents of A

关 键 词：中华鲟 后备亲本 海水养殖 血液生化指标 

分 类 号：S965.215[农业科学—水产养殖]