不同类型盐胁迫对小黑麦种子萌发的影响  

Effects of different salt stresses on triticale seed germination

作　　者：孟晨 鲁雪莉 王菊英 魏云冲 张成省 李义强[1,2,3] 徐宗昌 MENG Chen;LU Xue-li;WANG Ju-ying;WEI Yun-chong;ZHANG Cheng-sheng;LI Yi-qiang;XU Zong-chang(Marine Agriculture Research Center,Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266100,China;National Center of Technology Innovation for Comprehensive Utilization of Saline-alkali Land,Dongying 257345,China;Qingdao Key Laboratory of Coastal Saline-alkali Land Resources Mining and Biological Breeding,Qingdao 266100,China;College of Agriculture,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]中国农业科学院烟草研究所海洋农业研究中心,山东青岛266100 [2]国家盐碱地综合利用技术创新中心,山东东营257345 [3]青岛市滨海盐碱地资源挖掘与生物育种重点实验室,山东青岛266100 [4]青岛农业大学农学院,山东青岛266109

出　　处：《草业学报》2023年第12期171-180,共10页Acta Prataculturae Sinica

基　　金：中国农业科学院科技创新工程(CAAS-ZDRW202201,ASTIP-TRIC06);青岛市科技惠民示范引导专项(20-3-4-7-nsh)资助。

摘　　要：土壤盐渍化是制约农业生产的重要因素,小黑麦具有高产抗逆等特性,具有改良盐碱地的作用。以小黑麦为材料,以5种盐(NaCl,KCl,Na_(2)SO_(4),MgSO_(4),NaHCO_(3)),并设置10、25、50、100以及200 mmol·L^(-1)盐浓度梯度以及混合盐(NaHCO_(3)∶NaCl∶Na_(2)SO_(4)=1∶15∶84),设置0.05%,0.15%,0.30%,0.60%和1.20%盐浓度梯度对小黑麦进行盐胁迫处理,分析盐胁迫下小黑麦种子萌发阶段的发芽率、发芽势、发芽指数、活力指数及幼苗生长阶段的株高和根长,通过主成分分析及隶属函数分析对不同盐胁迫下小黑麦耐盐性进行综合评价。结果表明,小黑麦种子随着盐处理浓度的升高,其发芽率、发芽势、发芽指数和活力指数均逐渐降低,高浓度盐处理显著影响小黑麦种子的萌发。小黑麦幼苗株高及根长也随着盐处理浓度的增加而呈逐渐降低的趋势,其中NaHCO_(3)的抑制作用最显著。主成分分析表明,评价小黑麦耐盐性的主要指标为发芽率、发芽指数及根长。此外,隶属函数值排序表明,小黑麦对混合盐处理的耐受度相对较高,对NaHCO_(3)的耐受度最低。Soil salinization is an important factor that restricts agricultural production.Triticale has high yields and shows strong stress resistance,so it has the potential to improve saline-alkali land.In this study,triticale seeds were treated with five kinds of salts(NaCl,KCl,Na_(2)SO_(4),MgSO_(4),NaHCO_(3))at different concentrations(10,25,50,100 and 200 mmol·L^(-1))and compound salt solutions(NaHCO_(3)∶NaCl∶Na_(2)SO_(4)=1∶15∶84)at concentrations of 0.05%,0.15%,0.30%,0.60%and 1.20%(w/v).The germination rate,germination potential,germination index,vigor index,seedling length,and root length of triticale germinating seeds and seedlings under different salt stresses were analyzed.The salt tolerance of triticale under different salt stresses was comprehensively evaluated by a principal component analysis and membership function analysis.The results show that the germination rate,germination potential,germination index,and vigor index of triticale seeds decreased gradually as the concentration of each salt increased,and treatment with high concentrations of salts significantly inhibited the germination of the triticale seeds.The seedling length and root length of triticale seedlings also showed a decreasing trend with increasing salt concentrations.Among the salts,NaHCO_(3)had the strongest inhibitory effect.The results of the principal component analysis showed that the main indicators for evaluating the salt tolerance of triticale were germination rate,germination index,and root length.The ranking of membership function values showed that triticale had relatively high tolerance to mixed salt treatments and the lowest tolerance to NaHCO_(3).

关 键 词：小黑麦 盐胁迫 耐盐性分析 

分 类 号：S544.9[农业科学—作物学]