新型树脂BacT/Alert血液培养瓶抗菌药物吸附能力评估  

Evaluation of antibacterial drugs adsorption capacity of new resin BacT/Alert blood culture bottle

作　　者：罗璇[1] 闫少珍[1] 朱旭慧[1] 陈中举[1] LUO Xuan;YAN Shaozhen;ZHU Xuhui;CHEN Zhongju(Department of Clinical Laboratory,Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430010,China)

机构地区：[1]华中科技大学同济医学院附属同济医院检验科,湖北武汉430010

出　　处：《检验医学与临床》2023年第23期3429-3432,3437,共5页Laboratory Medicine and Clinic

基　　金：湖北省自然科学基金一般面上项目(2019CFB666)。

摘　　要：目的评估新型树脂BacT/Alert聚合物吸附珠血培养瓶(Bact/Alert APB需氧瓶,简称需氧瓶Bio-FA plus;Bact/Alert APB厌氧瓶,简称厌氧瓶Bio-FN plus)的抗菌药物吸附能力。方法采用体外模拟实验比较新型树脂BacT/Alert聚合物吸附珠血培养瓶(需氧瓶Bio-FA plus;厌氧瓶Bio-FN plus)和不含抗菌药物中和物质的BacT/Alert标准需氧瓶(简称需氧瓶Bio-SA)的阳性检出率和检出时间。抽取标准需氧瓶Bio-SA、新型树脂需氧瓶Bio-FA plus和厌氧瓶Bio-FN plus随机分为对照组和实验组,实验组血培养瓶接种磷酸缓冲盐溶液(PBS)和一定浓度的标准菌株菌悬液;对照组接种PBS、一定浓度的标准菌株菌悬液和一定浓度的临床常用抗菌药物溶液(头孢哌酮/舒巴坦、万古霉素、头孢唑啉、哌拉西林/他唑巴坦、米卡芬净、亚胺培南),记录并比较各组血培养瓶5 d内的阳性检出率及检出时间。结果实验组新型树脂需氧瓶Bio-FA plus的检出率为83.33%,厌氧瓶Bio-FN plus的检出率为100.00%,与标准需氧瓶Bio-SA检出率比较,差异有统计学意义(P<0.05);实验组新型树脂需氧瓶Bio-FA plus对头孢唑啉和哌拉西林/他唑巴坦的检出时间与对照组比较,差异均无统计学意义(P>0.05),实验组新型树脂需氧瓶Bio-FA plus对头孢哌酮/舒巴坦、万古霉素、米卡芬净的检出时间与对照组比较,差异均有统计学意义(P<0.05),差值按照从小到大的顺序分别是头孢哌酮/舒巴坦<万古霉素<米卡芬净,其对亚胺培南5 d内均未报阳。实验组新型树脂厌氧瓶Bio-FN plus对头孢哌酮/舒巴坦、哌拉西林/他唑巴坦和亚胺培南的检出时间与对照组比较,差异无统计学意义(P>0.05)。实验组新型树脂厌氧瓶Bio-FN plus对万古霉素和头孢唑啉的检出时间与对照组比较,差异均有统计学意义(P<0.05),差值分别为0.72 h和0.96 h。结论新型树脂BacT/Alert血培养瓶对所测试的抗菌药物具有较强的吸附能力,较需氧瓶BObjective To evaluate an antimicrobial drugs adsorption capacity of the new resin BacT/Alert polymer adsorption bead blood culture bottle(BactT/Alert APB aerobic bottle,referred to as aerobic bottle Bio-FA plus;BacT/Alert APB anaerobic bottle,referred to as anaerobic bottle Bio-FN plus).Methods In vitro simulation experiment was used to compare the positive detection rate and positive detection time between the new resin BacT/Alert polymer adsorbed bead blood culture bottle(aerobic bottle Bio-FA plus,anaerobic bottle Bio-FN plus)and the BacT/Alert standard aerobic bottle(referred to as aerobic bottle Bio-SA)without antibacterial neutralizing substances.Standard aerobic bottles Bio-SA,new resin aerobic bottles Bio-FA plus and anaerobic bottles Bio-FN plus were randomly divided into control group and experimental group.The experimental group was inoculated with phosphate buffer saline(PBS)and a certain concentration of standard strain suspension in blood culture bottles.The control group was inoculated with PBS,a certain concentration of standard strain suspension and a certain concentration of clinical common antibacterial drug solution(cefoperazone/sulbactam,vancomycin,cefazolin,piperacillin/tazobactam,micafunzin,imipenem).The positive detection rate and detection time of blood culture bottles within 5 d in each group were recorded and compared.Results In the experimental group,the detection rate of new resin aerobic bottle Bio-FA plus was 83.33%,and the detection rate of anaerobic bottle Bio-FN plus was 100.00%.Compared with the detection rate of standard aerobic bottle Bio-SA,the difference was statistically significant(P<0.05).There was no significant difference in the detection time of cefazolin and piperacillin/tazobactam in the new resin aerobic bottle Bio-FA plus between the experimental group and the control group(P>0.05).There were significant differences in the detection time of cefoperazone/sulbactam,vancomycin and micafungin in aerobic bottle Bio-FA plus between the experimental group and the control

关 键 词：新型树脂 血培养瓶 抗菌药物 吸附 

分 类 号：R446.5[医药卫生—诊断学]