血清PTX3、8-异前列腺素在哮喘-AECOPD的表达及与气道免疫抑制的关系  

Expression of serum PTX3 and 8-isoprostane in patients asthma-AECOPD and their relationship with airway immunosuppression

作　　者：黑雁 冯国平 宋根红[2] HEI Yan;FENG Guoping;SONG Genhong(Department of Respiratory and Critical Care,Yichuan County People's Hospital,Yan'an,Shaanxi 716200,China;Department of Respiratory Medicine,Affiliated Hospital of Yan'an University,Yan'an,Shaanxi 716200,China)

机构地区：[1]陕西省宜川县人民医院呼吸与危重症科,陕西延安716200 [2]陕西省延安大学附属医院呼吸内科,陕西延安716200

出　　处：《检验医学与临床》2023年第23期3526-3530,共5页Laboratory Medicine and Clinic

摘　　要：目的探讨血清正五聚蛋白3(PTX3)、8-异前列腺素(8-iso-PG)在哮喘-慢性阻塞性肺疾病(COPD)急性加重期(AECOPD)的表达及与气道免疫抑制的关系。方法选取2020年12月至2022年12月在陕西省宜川县人民医院治疗的哮喘-AECOPD患者60例作为急性加重组,哮喘-稳定期COPD患者55例作为稳定组,另选取同期健康体检者30例作为健康组。检查所有受试者血清8-iso-PG、PTX3水平及相关免疫指标水平[CD3^(+)、CD4^(+)、CD8^(+)T淋巴细胞及自然杀伤细胞(NK细胞)比例];比较不同组中血清PTX3、8-isoPG及相关免疫指标水平;采用受试者工作特征(ROC)曲线评估血清8-iso-PG、PTX3对哮喘-AECOPD的诊断效能。采用Pearson相关分析血清PTX3、8-iso-PG与免疫指标的关系。结果3组血清PTX3、8-iso-PG水平及CD8^(+)T淋巴细胞比例比较,急性加重组>稳定组>健康组,差异均有统计学意义(P<0.05);3组CD3^(+)、CD4^(+)、CD8^(+)T淋巴细胞及NK细胞比例比较,急性加重组<稳定组<健康组,差异均有统计学意义(P<0.05)。血清PTX3、8-iso-PG诊断哮喘-AECOPD的曲线下面积(AUC)分别为0.946、0.908(P<0.05)。根据血清PTX3、8-iso-PG的ROC曲线分析结果的最佳截断值将急性加重组分为PTX3<7.233 ng/L组、PTX3≥7.233 ng/L组、8-iso-PG<8.018 ng/mL组、8-iso-PG≥8.018 ng/mL组。PTX3≥7.233 ng/L组CD3^(+)、CD4^(+)T淋巴细胞及NK细胞比例明显低于PTX3<7.233 ng/L组,CD8^(+)T淋巴细胞比例高于PTX3<7.233 ng/L组,差异均有统计学意义(P<0.05);8-iso-PG≥8.018 ng/mL组CD3^(+)、CD4^(+)T淋巴细胞及NK细胞比例明显低于8-iso-PG<8.018 ng/mL组,CD8^(+)T淋巴细胞比例高于8-iso-PG<8.018 ng/mL组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,血清PTX3、8-iso-PG与CD8^(+)均呈正相关(P<0.05),与CD3^(+)、CD4^(+)、NK细胞比例均呈负相关(P<0.05)。结论血清PTX3、8-iso-PG在哮喘-AECOPD患者中表达水平会明显升高,血清PTX3、8-iso-PG水平与哮喘-AECOPD患者免疫抑�Objective To investigate the expression of serum pentraxin 3(PTX3)and 8-iso-prostaglandin(8-iso-PG)in asthma chronic obstructive pulmonary disease(COPD)acute exacerbation(AECOPD)and their relationship with airway immunosuppression.Methods A total of 60 asthma-AECOPD patients treated in Yichuan County People's Hospital of Shaanxi Province from December 2020 to December 2022 were selected as acute plus group,55 asthma-stable COPD patients were selected as stable group,and 30 healthy physical examination subjects were selected as healthy group.Serum 8-iso-PG,PTX3 and immune indicators[CD3^(+),CD4^(+),CD8^(+)T lymphocytes and natural killer cell(NK cell)]were detected in all subjects.Serum PTX3,8-iso-PG and immune indexes levels were compared among the different groups.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy of serum PTX3 and 8-iso-PG in the diagnosis of asthma-AECOPD.Pearson correlation was used to analyze the relationship between serum PTX3,8-iso-PG and immune indexes.Results The levels of serum PTX3,8-iso-PG and the proportion of CD8^(+)T lymphocytes in the 3 groups were compared,and the acute plus group was>stable group>healthy group,and the differences were statistically significant(P<0.05).The proportions of CD3^(+),CD4^(+),CD8^(+)T lymphocytes and NK cells in the 3 groups were compared,and the acute plus group<stable group<healthy group,and the differences were statistically significant(P<0.05).The area under the curve(AUC)of serum PTX3 and 8-iso-PG in the diagnosis of asthma-AECOPD was 0.946 and 0.908,respectively(P<0.05).According to the optimal cut-off value of ROC curve analysis results of the serum PTX3 and 8-iso-PG,the patients of the acute plus group were divided into PTX3<7.233 ng/L group,PTX3≥7.233 ng/L group,8-iso-PG<8.018 ng/mL group and 8-iso-PG≥8.018 ng/mL group.The proportions of CD3^(+),CD4^(+)T lymphocytes and NK cells in the PTX3≥7.233 ng/L group were lower than those in the PTX3<7.233 ng/L group,and the proportion of CD8^(+)T lymphocytes

关 键 词：正五聚蛋白3 8-异前列腺素 重叠综合征 免疫抑制 相关性 

分 类 号：R563[医药卫生—呼吸系统]