基于ITS和ISSR的灵芝种质资源遗传多样性分析  

Genetic Diversity Analysis of Ganoderma lucidum Germplasm Resources Based on ITS and ISSR

作　　者：许琳 张瑞 王胜 李金涛 刘琳玲[1] 闫梅霞[1] XU Lin;ZHANG Rui;WANG Sheng;LI Jintao;LIU Linling;YAN Meixia(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China;College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《河南农业科学》2023年第11期66-74,共9页Journal of Henan Agricultural Sciences

基　　金：吉林省科技发展计划项目(20210401101YY)。

摘　　要：为明确吉林栽培的灵芝(Ganoderma lucidum)种质资源的遗传多样性,以吉林省18个主栽灵芝菌株为试验材料,通过应用ITS(Internal transcribed spacer)序列分析及拮抗试验,并与ISSR(Inter-simple sequence repeat)分子标记相结合对其进行鉴定和遗传多样性分析。基于ITS序列构建的系统发育树表明,18种灵芝菌株均与赤芝(Ganoderma lingzhi)聚为一类,并细分为4个类群;79.7%的灵芝菌株之间存在拮抗现象,基于ITS序列分析及拮抗试验结果,菌株被分为7个类群;ISSR遗传多样性分析结果表明,18种灵芝菌株的遗传相似系数在0.3824~0.9744,平均为0.6529,当相似系数约0.679时,其亦可被分为7个类群。综上,基于ISSR分子标记的分类结果与基于ITS序列分析+拮抗试验结果的分类一致,18种灵芝菌株表现出明显的遗传多样性。In order to clarify the genetic diversity of Ganoderma lucidum germplasm resources cultivated in Jilin Province,18 main Ganoderma lucidum strains in Jilin Province were used as test materials,the internal transcribed spacer sequence analysis and antagonism test were applied,and inter⁃simple sequence repeat molecular markers were combined for identification and genetic diversity analysis.The phylogenetic tree based on ITS sequence showed that all the 18 Ganoderma lucidum strains were grouped together with Ganoderma lingzhi,and were subdivided into four groups.Antagonism was found among 79.7%of Ganoderma lucidum strains.The strains were divided into seven groups based on ITS sequence analysis and antagonism test results.The results of ISSR genetic diversity analysis showed that the genetic similarity coefficient of 18 Ganoderma lucidum isolates ranged from 0.3824 to 0.9744,with an average of 0.6529.When the similarity coefficient was about 0.679,the isolates could be divided into seven groups.In summary,the classification results based on ISSR molecular markers were consistent with the classification results based on ITS sequence analysis+antagonism test,and 18 ganoderma lucidum strains showed obvious genetic diversity.

关 键 词：灵芝 拮抗试验 ITS ISSR 遗传多样性 

分 类 号：S567.31[农业科学—中草药栽培]