基于GC-MS及主成分综合评价广东不同产地益智挥发油成分差异  

Comprehensive Evaluation of Alpinia oxyphylla Volatile Oil Components in Different Producing Areas of Guangdong Based on GC-MS and Principal Component Analysis

作　　者：陈卫明 范会云 黄勇 谢黎黎 梁秋玲 曾庆钱 郑艳 吴江祝 郑良豹 CHEN Wei-ming;FAN Hui-yun;HUANG Yong(Guangdong Institute of Traditional Chinese Medicine,Guangzhou,Guangdong 510640)

机构地区：[1]广东省中药研究所,广东广州510640 [2]广东农垦热带作物科学研究所,广东茂名525100

出　　处：《安徽农业科学》2023年第23期173-177,共5页Journal of Anhui Agricultural Sciences

基　　金：广东省重点领域研发计划项目(2020B020221001);广东省中医局科研项目(20211065);2021年广东省科技专项-农村科技特派员驻镇帮镇扶村组团式帮扶项目。

摘　　要：[目的]比较广东5个主产地的益智挥发油成分的差异性,为益智的合理利用和资源开发提供参考。[方法]采用水蒸气蒸馏法对不同产地的益智挥发油进行提取,用GC-MS法对所得挥发油组分进行鉴定分析,同时进行谱库检索,并通过SIMCA+P统计软件进行主成分分析。[结果]广东5个主产地的益智果实挥发油含量均大于1.00%;采用GC-MS技术共鉴定出58个化合物,共有化合物53个,其中γ-松油烯的含量最高;利用主成分分析将53个指标简化为4个综合指标,主成分综合得分从高到低排序为高州、阳春、罗定、信宜、化州;聚类分析表明,5份益智样品在欧氏距离d=8.00的水平上,可划分为3大类群。[结论]广东省5个不同产地益智挥发油化学成分在组成和含量上存在一定的差异。[Objective]To compare the differences of volatile oil components of A.oxyphylla from five main producing areas in Guangdong,and to provide reference for rational utilization and resource development of A.oxyphylla.[Method]The volatile oil from A.oxyphylla from different habitats was extracted by steam distillation,and the components of the volatile oil were identified and analyzed by GC-MS.At the same time,the spectral database was searched,and the principal component analysis was performed by SIMCA+P statistical software.[Result]The contents of volatile oil in A.oxyphylla fruits from five main producing areas in Guangdong were higher than 1.00%.A total of 58 compounds were identified by GC-MS,including 53 compounds,and the content ofγ-terpinolene was the highest.The 53 indexes were simplified into 4 comprehensive indexes by principal component analysis,and the comprehensive scores of principal components from high to low were as follows:Gaozhou>Yangchun>Luoding>Xinyi>Huazhou.Cluster analysis showed that five A.oxyphylla samples could be divided into three groups at the level of Euclidean distance d=8.00.[Conclusion]There are some differences in the chemical constituents and contents of essential oils of A.oxyphylla from five different producing areas in Guangdong Province.

关 键 词：益智 挥发油 GC-MS 主成分分析 聚类分析 

分 类 号：R932[医药卫生—生药学]