基于EST-SSR标记的金线莲叶色突变体分子身份分析  

Identification of Molecular ID of Leaf Color Mutants in Anoectochilus roxburghii by EST-SSR Molecular Markers

作　　者：樊荣辉[1] 陈艺荃 钟淮钦[1] 叶秀仙[1] Fan Ronghui;Chen Yiquan;Zhong Huaiqin;Ye Xiuxian(Institute of Crop Sciences,Fujian Academy of Agricultural Science/Fujian Engineering Research Centerfor Characteristic Floriculture,Fuzhou,Fujian 350013,China;Institute of Agricultural Enginering Technogy Fujan Academy of Agicultural Science Fuzhou Fujian 35003,China)

机构地区：[1]福建省农业科学院作物研究所/福建省特色花卉工程技术研究中心,福建福州350013 [2]福建省农业科学院农业工程技术研究所,福建福州350003

出　　处：《东南园艺》2023年第4期281-285,共5页Southeast Horticulture

基　　金：福建省自然科学基金项目(2020J011370);福建省农业科学院科技创新团队建设项目(CXTD2021010-2);福建省人民政府与中国农业科学院高质量发展超越“5511”协同创新工程项目(XTCXGC2021003)。

摘　　要：【目的】为金线莲(Anoectochilus roxburghii)从DNA分子水平上探索叶色突变体的遗传差异。【方法】采用EST-SSR分子标记技术对金线莲及其2个叶色突变体进行分子鉴定。【结果】20对SSR引物共扩增出78条谱带,其中3对引物(JXL-6、JXL-14和JXL-16)能稳定扩增出差异条带,多态性条带9条,多态性比率11.54%。【结论】获得的2个叶色突变体为金线莲突变体,叶色突变体与亲本在DNA分子水平差异较小。【Objective】This study aimed to explore genetic differences of leaf color mutants of Anoectochilus roxburghii at the DNA level.【Method】Molecular identification of Anoectochilus roxburghi and its two leaf color mutants was conducted by EST-SSR molecular markers.【Result】The results showed that 20 pairs of SSR primers produced 78 bands,3 pairs of primers(JXL-6,JXL-14 and JXL-16)could stably amplify differential bands.Nine bands were polymorphic produced in 20 pairs of SSR primers and polymorphism ratio was 11.54%.【Conclusion】Two leaf color mutants were identified as mutants of Anoectochilus roxburghi.However,there were small differences between mutants and parents at DNA molecular level.

关 键 词：金线莲 自然突变 分子标记鉴定 SSR 

分 类 号：S567.239[农业科学—中草药栽培]