光催化TiO_(2)-NaOH预处理稻秆协同酶法制备葡萄糖研究  

Study on Photocatalytic TiO_(2)-NaOH Pretreatment of Rice Straw for Synergistic Enzymatic Preparation of Glucose

作　　者：潘亮 毕艳红 迟永洲 罗思 袁向阳 王朝宇 赵祥杰 PAN Liang;BI Yan-hong;CHI Yong-zhou;LUO Si;YUAN Xiang-yang;WANG Zhao-yu;ZHAO Xiang-jie(School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huai′an 223003,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003

出　　处：《化学试剂》2023年第12期77-83,共7页Chemical Reagents

基　　金：国家自然科学基金资助项目(21676114);江苏省研究生实践创新计划项目(SJCX23-1853);江苏省市场监督管理局科技项目(KJ21125104);淮阴工学院2023大学生创新创业训练计划项目(202311049477YJ)。

摘　　要：采用光催化耦合TiO_(2)-NaOH预处理稻秆的方法,以有效提高其酶解糖化的效果。研究了光催化剂TiO_(2)粒径、料液比、TiO_(2)质量浓度和光催化时间对稻秆预处理的影响。采用三因素三水平的Box-Behnken试验设计,利用响应面法对稻秆预处理工艺参数进行研究,以酶解预处理稻秆的葡萄糖质量浓度为响应值进行响应面分析,得到最优光催化条件:料液比为1∶41(g/mL),P25型TiO_(2)质量浓度为0.7 g/L,光催化时间为54 min。在此条件下,酶解后葡萄糖质量浓度最高达(19.02±0.16)mg/mL。所建立的TiO_(2)-NaOH预处理方法,为木质纤维素的高效预处理提供了借鉴,对推动生物质能源的高效利用提供了理论依据。A photocatalytic coupled TiO_(2)-NaOH pretreatment method was used to improve the enzymatic hydrolysis of rice stalks.The effects of TiO_(2)particle size,solid-liquid ratio,TiO_(2)mass concentration and photocatalytic time on rice stalks were studied.A three-factor and three-level Box-Behnken experimental scheme was adopted to explore the parameters of straw pretreatment process by response surface method.The optimal photocatalytic conditions were obtained by response surface analysis with glucose mass concentration as the response value:solid-liquid ratio was 1∶41(g/mL),P25 TiO_(2)mass concentration was 0.7 g/L,and photocatalytic time was 54 min.The highest glucose mass concentration was(19.02±0.16)mg/mL after enzymolysis.The TiO_(2)photocatalytic combined with NaOH pretreatment method established in this study provides a reference for the efficient pretreatment of lignocellulose and a theoretical basis for promoting the utilization of biomass energy.

关 键 词：光催化 TiO_(2)光催化剂 木质纤维素 酶解 生物质能源 

分 类 号：Q556[生物学—生物化学]