重组酶扩增技术概述及应用研究进展  

Overview of Recombinase Amplification Technology and Its Application

作　　者：陈兴浩 李鑫[2] 沈海潇 张校培 徐丽娜 张鹤平 葛菲菲[2] 杨显超[2] 刘健[2] 白艺兰 王建[2] 杨德全[2] 赵洪进[2] Chen Xinghao;Li Xin;Shen Haixiao;Zhang Xiaopei;Xu Lina;Zhang Heping;Ge Feifei;Yang Xianchao;Liu Jian;Bai Yilan;Wang Jian;Yang Dequan;Zhao Hongjin(College of Life Science and Food Engineering,Hebei University of Engineering,Handan,Hebei 056038,China;Shanghai Center for Animal Disease Control and Prevention,Shanghai 201103,China;College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui 230031,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]上海市动物疫病预防控制中心,上海201103 [3]安徽农业大学动物科技学院,安徽合肥230031

出　　处：《中国动物检疫》2023年第11期76-81,共6页China Animal Health Inspection

基　　金：上海市科技兴农项目(沪农科推字[2022]第2-1号)。

摘　　要：重组酶扩增技术是近几年快速发展起来的一类等温扩增技术,相对于传统的核酸检测方法,具有设备要求低、操作简单等优势,在现场快速检测方面有着广阔的应用前景。本文简要介绍了重组酶扩增技术的技术原理、优点和难点,重点阐述了其在病毒、细菌、寄生虫及其他微生物检测领域的应用研究进展,旨在为重组酶扩增技术的进一步发展与应用提供参考。With continuous development of animal husbandry,the diagnosis of animal diseases has become increasingly important,especially early diagnosis as a key to prevent and treat animal diseases.Compared with traditional nucleic acid detection,recombinase amplification technology is a kind of isothermal amplification technology developed rapidly in recent years with the advantages of low requirements for equipment as well as easy operation,in particular with broad application prospect in field rapid detection.In the paper,the technical principle,advantages and difficulties of the technology were briefly introduced,focusing on research progress of its application in the detection of viruses,bacteria,parasites and other microorganisms,with a view to providing a reference for its furtherdevelopment and application.

关 键 词：重组酶聚合酶扩增技术 酶促重组等温扩增技术 重组酶介导等温扩增技术 多酶恒温核酸快速扩增技术 

分 类 号：S854.4[农业科学—临床兽医学]