奶山羊乳房炎病原菌分离鉴定与药敏检测分析  

Isolation and Identification of Pathogenic Bacteria from Dairy Goat Mastitis and Analysis of Drug Sensitivity

作　　者：郭东芳 GUO Dongfang(Xinxiang County Animal Quarantine Station,Xinxiang Henan 453700)

机构地区：[1]新乡县动物检疫站,河南新乡453700

出　　处：《中国乳业》2023年第10期67-71,共5页China Dairy

摘　　要：[目的]调查新乡县奶山羊乳房炎病原菌和耐药性。[方法]对患乳房炎奶山羊283份乳汁病料进行病原菌分离培养、生化试验,利用细菌16S rRNA通用引物PCR扩增,采用Kirby-Bauer纸片法对致病菌进行9种抗生素耐药性检测分析。[结果]共分离鉴定出92株致病菌,其中,23株为金黄色葡萄球菌,占25.0%;11株为无乳链球菌,占11.96%;4株为化脓链球菌,占4.35%;12株为乳房链球菌,占13.04%;17株为大肠埃希氏菌,占18.48%;25株为其他菌株,占27.17%。致病菌对9种抗生素产生不同程度耐药性,金黄色葡萄球菌对阿莫西林、克林霉素、头孢哌酮、红霉素的耐药率分别为65.22%、69.56%、69.57%、60.87%,链球菌对卡那霉素耐药率为81.48%,大肠埃希氏菌对克林霉素、卡那霉素耐药率为70.59%、76.47%。[结论]金黄色葡萄球菌、链球菌和大肠埃希氏菌是奶山羊乳房炎主要致病菌,致病菌株耐药性较严重。[Objective]To investigate the pathogenic bacteria and drug resistance of mastitis in dairy goats in Xinxiang County.[Method]283 milk samples of dairy goats with mastitis were isolated and cultured,biochemical tests were conducted,and 16S rRNA universal primer PCR amplification was used to amplify the pathogenic bacteria,and K-B method was used to detect and analyze nine kinds of antibiotic resistance of the pathogenic bacteria.[Result]92 strains of pathogenic bacteria were isolated and identified,of which 23 strains were Staphylococcus aureus,accounting for 25.0%,11 strains were Streptococcus agalactis,accounting for 11.96%,4 strains were Streptococcus pyogenes,accounting for 4.35%,12 strains were Streptococcus uberis,accounting for 13.04%,17 strains were Escherichia coli,accounting for 18.48%,and 25 strains were other strains,accounting for 27.17%.The pathogenic bacteria have different degrees of resistance to nine antibiotics.The resistance rates of Staphylococcus aureus to Amoxicillin,Clindamycin,Cefoperazone,and Erythromycin are 65.22%,69.56%,69.57%,60.87%,respectively.The resistance rate of Streptococci to Kanamycin is 81.48%,and that of Escherichia coli to Clindamycin and Kanamycin are 70.59%and 76.47%.[Conclusion]Staphylococcus aureus,Streptococcus and Escherichia coli were the main pathogenic bacteria of dairy goat mastitis,and the drug resistance of the pathogenic bacteria was serious.

关 键 词：奶山羊 乳房炎 病原菌 分离鉴定 耐药性 

分 类 号：S858.27[农业科学—临床兽医学]